Abstract: In this paper, we studied differences of immature embryo calli, proliferation, differentiation and glyphosate sensitivity in different genotypes by using the immature embryo of six wheat ( Zhengmai 9023,Zhengmai 366,Zhoumai18,Aikang 58,Huapei NO.5 and Huapei NO.8)cultivars as explants.The results showed that the cal-lus formation time and initial time of differentiation had a significant difference in different wheat cultivars, Huapei NO.5 and Huapei NO.8 were optimal for tissue culture because the index of callus formation time and initial time were higher than others, regenerated plantlet yield reached high level.Screening of wheat varieties to glyphosate sen-sitivity through 0,1,2,3,4 mmol/L 5 kinds of glyphosate concentration, results showed differentiation and Callus Browning death compared to more obvious, is the ideal screening period.The concentration of 3 mmol/L Zhengmai 9023, Zhoumai 18, Zhengmai 366 differentiation rate compared with the control group showed significant differ-ences,but no significant difference with 4 mmol/L.Aikang 58 in the screening concentration of glyphosate compared with control differentiation rate decreased gradually, in 4 mmol/L of glyphosate screening differentiation rate reached 2.08%,most of the callus growth was inhibited.For Huapei No.5,Huapei No.8 and other varieties compared to more sensitive to glyphosate, screening concentration was 2 mmol/L,when compared with the control differentiation rate decreased,3.42%and 4.17%,respectively.

Key words: ImmatuRe embRyos of wheat, Calli, DiffeRentiation, Glyphosate, Sensitivity

CLC Number: 

• S512.1