Genetic PolymoRphism of 37 Onion GeRmplasm ResouRces as Revealed by RAPDs and SSRs MaRkeRs

doi:10.7668/hbnxb.2013.06.020

Abstract

Abstract: In this paper,the genetic diversity of 37 onions(Allium cepa L.) germplasm resources were analyzed by SSRs and RAPDs markers and the results of these two methods were compared.The results showed that 61 poly-morphic bands were detected by 15 RAPD primers and 41 polymorphic bands were detected by 8 SSR primers.Both RAPDs and SSRs were highly polymorphic.The percentage of polymorphic RAPDs bands was 84.7%.The average effective number of alleles was 3.75 for SSRs.The average effective number of alleles, Nei's genetic diversity index, Shannon information index for SSRs markers were higher than those in RAPDs.UPGMA cluster analysis indicated that both the two markers could divided 37 germplasm into four groups and the classification were fundamentally i-dentical.The study suggested that both RAPDs and SSRs could be used to analyze the genetic diversity of onion germplasm resources, but the effect for SSRs marker was better than RAPDs.

Key words: Onion, RAPD, SSR, Genetic diveRsity

CLC Number:

S633.2

ZHAI Ya-hui, MA Rong-li, CHENG Yan, WU Hai-tao, ZHANG Guang-xing. Genetic PolymoRphism of 37 Onion GeRmplasm ResouRces as Revealed by RAPDs and SSRs MaRkeRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 115-120. doi: 10.7668/hbnxb.2013.06.020.

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http://www.hbnxb.net/EN/Y2013/V28/I6/115

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