Abstract:

In order to deeply understand the genetic variation of yield related traits of flax germplasm,229 flax germplasm resources were used as materials,SSR markers that were significantly associated with yield related traits in flax were explored by association analysis of generalized linear model(GLM)and mixed linear model(MLM). The results showed that the indexes of plant height((58.40±5.67)cm),fruit number per plant(17.50±3.50)and grain weight per plant((0.55±0.15)g)of flax germplasm planted in Hohhot were the largest under four environments. In Jining area,the indexes of branch number(5.00±1.25),fruit grain number(6.52±2.67)and 1000-seed weight((5.58±1.48)g)were the largest. The phenotypic variation coefficients of the seven yield related traits were 11.18%—18.29%. The order of generalized heritability was 1000-seed weight > seeds per capsule>seeds weight per plant>plant height>capsule number per plant>branch number>stem length. When the population structure K=4,229 flax germplasm were divided into 4 groups. A total of 365 bands were amplified by 30 pairs of SSR primers,and 36 SSR markers were detected by GLM correlation analysis with 7 yield related traits of flax. The interpretation rate of phenotypic variation was 1.15%(Lu146)—7.75%(Lub747);MLM association analysis detected 23 SSR markers,and the interpretation rate of phenotypic variation was 2.26%(Lu203a)—7.16%(Lu291). Under GLM and MLM,Lu203a and Lua125a markers were detected on chromosome 12 and 2,respectively. It was concluded that SSR markers in flax could be well correlated with yield-related traits and had a certain explanatory rate.

Key words: Flax, Yield, Correlation analysis, SSR