Effects of Chlorate Treatment on Traits Related to Different Barley Landraces at the Seedling Stage

doi:10.7668/hbnxb.20193128

Abstract

Abstract:

In order to establish a chlorate-based screening system for barley with different nitrogen use efficiency(NUE),the effects of chlorate treatment on barley seedlings were explored,barley germplasms with different responses to chlorate treatment will be used for molecular mechanism research on NUE and crop breeding with high NUE in barley.Eighteen barley landraces from Shanghai region and three main cultivars in present barley production were used as test materials,and hydroponic experiments were carried out in an artificial climate room,and 2 mmol/L KNO₃(CK)and 2 mmol/L KClO₃ treatments were setrespectively.The traits of seedling height,root length,shoot and root dry masses of barley seedlings were measured after 4 days of treatments,respectively.It was showed that the growth of barley seedlings was obviously inhibited under chlorate treatment,especially the seedling height and dry mass related traits.The ANOVA showed that there were extremely significant differences in all traits under both chlorate treatment and normal condition(the control),and there were also extremely significant interactions between the treatments and varieties.The coefficient of variation analysis showed that the coefficients of variation of root length and dry mass related traits were relatively large in both treatments,while the seedling height was relatively small.Correlation analysis also showed that these indexes were positively correlated under both conditions.The chlorate susceptibility was consistent based on both seedling height and shoot dry mass in general,but there were still some differences.The response of seedling height and shoot dry mass to chlorate could be used as an index for screening barley with different NUE,and B104,B002,B053,B092 and B068 were among the six most sensitive varieties to chlorate based on both seedling height and shoot dry mass,indicating that these five barley cultivars might have higher NUE;while B016,B006 and B008 were among the 6 least sensitive varieties to chlorate,suggesting that the NUE of these three barley varieties might be relatively lower.

Key words: Hordeum vulgare L., Shoot dry mass, Seedling height, Chlorate sensitivity

LI Luli, ZONG Yingjie, YANG Hua, XU Hongwei, GAO Runhong, LU Ruiju, LIU Chenghong, CHEN Zhiwei. Effects of Chlorate Treatment on Traits Related to Different Barley Landraces at the Seedling Stage[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 90-95. doi: 10.7668/hbnxb.20193128.

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References 26

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编号 Code	品种名称 Variety name	编号 Code	品种名称 Variety name	编号 Code	品种名称 Variety name
B002	紫大麦	B027	红三月黄大麦	B092	白柱头元麦
B006	罗次红芒大麦	B046	晚大麦	B098	红筋六柱头
B008	沪麦1号	B053	奉贤早大麦	B104	白三月黄
B011	本大麦	B067	大麦	B107	上海四柱头
B014	红大麦	B068	六柱头紫大麦	花11	花11
B016	红筋二棱大麦	B074	宝山老脱须	花22	花22
B025	上海三月黄大麦	B086	上海灯笼大麦	花30	花30

编号 Code	品种名称 Variety name	编号 Code	品种名称 Variety name	编号 Code	品种名称 Variety name
B002	紫大麦	B027	红三月黄大麦	B092	白柱头元麦
B006	罗次红芒大麦	B046	晚大麦	B098	红筋六柱头
B008	沪麦1号	B053	奉贤早大麦	B104	白三月黄
B011	本大麦	B067	大麦	B107	上海四柱头
B014	红大麦	B068	六柱头紫大麦	花11	花11
B016	红筋二棱大麦	B074	宝山老脱须	花22	花22
B025	上海三月黄大麦	B086	上海灯笼大麦	花30	花30

来源 Source	性状 Trait	自由度 df	均方 MS	F值 F value	显著性 Significance
处理	苗高	1	6 250.361	870.560	0.000
Treatment	根长	1	5 120.292	504.698	0.000
	地上部干质量	1	0.016	150.880	0.000
	根干质量	1	0.006	394.966	0.000
品种	苗高	20	51.399	2.621	0.000
Variety	根长	20	61.551	3.138	0.000
	地上部干质量	20	0.001	5.485	0.000
	根干质量	20	0.000	1.796	0.024
处理×品种	苗高	20	32.202	8.248	0.000
Treatment×Variety	根长	20	41.104	6.637	0.000
	地上部干质量	20	0.000	9.178	0.000
	根干质量	20	0.000	8.074	0.000
误差	苗高	410	3.904
Error	根长	410	6.193
	地上部干质量	168	0.000
	根干质量	168	0.000

来源 Source	性状 Trait	自由度 df	均方 MS	F值 F value	显著性 Significance
处理	苗高	1	6 250.361	870.560	0.000
Treatment	根长	1	5 120.292	504.698	0.000
	地上部干质量	1	0.016	150.880	0.000
	根干质量	1	0.006	394.966	0.000
品种	苗高	20	51.399	2.621	0.000
Variety	根长	20	61.551	3.138	0.000
	地上部干质量	20	0.001	5.485	0.000
	根干质量	20	0.000	1.796	0.024
处理×品种	苗高	20	32.202	8.248	0.000
Treatment×Variety	根长	20	41.104	6.637	0.000
	地上部干质量	20	0.000	9.178	0.000
	根干质量	20	0.000	8.074	0.000
误差	苗高	410	3.904
Error	根长	410	6.193
	地上部干质量	168	0.000
	根干质量	168	0.000

品种 Varieties	苗高/cm Seedling height		根长/cm Root length		地上部干质量/g Shoot dry mass		根干质量/g Root dry mass
品种 Varieties	对照CK	处理Treatment	对照CK	处理Treatment	对照CK	处理Treatment	对照CK	处理Treatment
B002	22.60±1.61a	12.87±0.58b	11.17±2.24c	5.81±1.91d	0.039 8±0.005 1e	0.020 4±0.002 1f	0.016 7±0.003 7g	0.007 2±0.002 3h
B006	18.33±1.15a	12.95±1.68b	11.09±2.79c	8.30±2.37d	0.033 2±0.002 8e	0.027 4±0.001 8f	0.013 7±0.001 4g	0.008 2±0.000 8h
B008	21.82±2.12a	16.94±1.72b	7.99±2.84c	6.13±2.04c	0.052 2±0.003 8e	0.040 8±0.003 2f	0.014 2±0.001 9g	0.009 3±0.001 3h
B011	24.14±3.75a	16.06±2.64b	14.71±4.64c	7.19±2.77d	0.063 9±0.007 7e	0.042 9±0.004 3f	0.021 1±0.003 0g	0.008 8±0.001 8h
B014	19.68±2.36a	11.79±1.69b	11.34±3.96c	6.13±1.80d	0.044 1±0.006 1e	0.031 1±0.005 6f	0.017 1±0.003 3g	0.007 6±0.001 2h
B016	17.57±2.36a	16.08±2.10a	7.84±2.33c	5.75±2.16c	0.032 5±0.003 9e	0.038 0±0.005 7e	0.011 3±0.002 1g	0.009 7±0.001 2g
B025	20.42±1.41a	14.05±2.09b	11.05±2.63c	5.77±2.14d	0.044 8±0.003 2e	0.035 4±0.002 3f	0.013 8±0.001 9g	0.007 6±0.001 3h
B027	17.36±2.35a	11.80±0.84b	10.01±2.13c	3.98±1.05d	0.038 4±0.001 4e	0.027 7±0.001 8f	0.013 4±0.001 7g	0.008 5±0.001 5h
B046	22.90±2.06a	15.10±1.49b	13.92±3.08c	6.02±2.34d	0.058 7±0.001 2e	0.032 7±0.001 5f	0.019 9±0.001 4g	0.006 9±0.000 7h
B053	24.05±1.50a	14.83±1.54b	15.73±3.04c	2.98±0.88d	0.056 5±0.002 9e	0.025 4±0.004 6f	0.017 3±0.002 0g	0.004 0±0.000 2h
B067	23.70±3.04a	15.38±1.37b	13.82±2.05c	7.06±2.21d	0.047 0±0.010 2e	0.027 6±0.005 2f	0.020 1±0.004 5g	0.005 8±0.001 2h
B068	24.29±2.54a	14.81±2.43b	15.07±3.15c	6.75±1.59d	0.060 1±0.008 5e	0.031 3±0.002 8f	0.020 5±0.003 5g	0.007 7±0.002 1h
B074	22.95±1.64a	14.45±1.35b	14.21±2.22c	7.12±2.15d	0.044 5±0.003 8e	0.031 6±0.003 8f	0.019 5±0.002 9g	0.009 4±0.002 5h
B092	24.75±1.80a	13.13±1.30b	13.23±3.08c	3.43±1.46d	0.050 1±0.006 6e	0.028 1±0.002 4f	0.017 6±0.002 9g	0.004 8±0.000 6h
B086	22.15±2.96a	15.03±2.72b	13.27±3.31c	5.65±3.31d	0.045 9±0.005 8e	0.034 5±0.004 1f	0.015 0±0.001 7g	0.005 7±0.001 5h
B098	24.80±1.96a	15.92±1.37b	9.56±2.35c	5.45±1.67d	0.052 8±0.003 5e	0.043 0±0.003 9f	0.018 6±0.001 8g	0.008 6±0.001 5h
B104	25.26±2.42a	13.80±2.10b	15.98±2.43c	4.56±1.36d	0.059 8±0.007 4e	0.030 7±0.002 5f	0.020 5±0.003 1g	0.006 8±0.001 4h
B107	21.55±1.92a	13.81±1.73b	11.53±1.97c	5.92±2.43d	0.055 7±0.006 5e	0.033 9±0.003 8f	0.019 8±0.002 2g	0.007 3±0.002 5h
花11	20.82±1.72a	14.93±1.30b	15.55±2.71c	7.96±3.06d	0.066 7±0.005 8e	0.045 3±0.004 4f	0.028 3±0.004 9g	0.010 5±0.002 3h
花22	20.01±1.54a	16.50±2.38b	11.69±3.10c	5.05±1.25d	0.065 5±0.007 1e	0.046 5±0.005 2f	0.023 4±0.002 6g	0.011 1±0.001 4h
花30	21.60±1.24a	15.32±0.98b	17.85±1.76c	8.86±1.91d	0.074 9±0.004 2e	0.045 2±0.005 2f	0.026 8±0.001 6g	0.011 5±0.001 3h
标准差	2.38	1.45	2.69	1.50	0.011 3	0.007 4	0.004 3	0.002 0
Standard deviation
平均值 Mean	21.05	13.95	12.24	5.79	0.049 9	0.033 0	0.017 9	0.007 7
变异系数/%	11.30	10.39	21.97	25.93	22.72	22.29	24.15	25.46
Coefficient variation

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