Variation Analysis of Heading Date Alleles Using Rice Single Segment Substitution Lines

doi:10.7668/hbnxb.20192258

Abstract

Abstract: In order to study the reasons for the difference of rice heading date between SSSL (Single segment substitution line) and acceptor parent HJX74 (Huajingxian 74), used HJX74 and five SSSLs WY18 (W06-15-18-3-11), WY24 (W08-16-3-2), WY26 (W11-15-7-1-1), WY60 (W15-3-1-38) and WY61 (W17-10-5-5-35-9-2) with significant difference in heading date as materials, cloned the known heading date alleles on their substitution fragments, obtained their CDS sequence, and used a variety of bioinformatics methods to perform sequence alignment, including CDS sequences and amino acid sequences, compared the similarities and differences of amino acid physicochemical properties and expression differences. According to the phenotype of heading date in the field, WY18, WY60 and WY61 showed late heading date compared with HJX74, while WY24 and WY26 showed early heading date. Compared with HJX74, there were 4 SNP differences in the heading date allele DTH8 on the WY18 substitution fragment, 7 SNP differences in WY26 OsDof12, and 6 SNP differences in WY60 DTH8. However, none of the above SNP mutations changed the hydrophilicity and physicochemical properties of its amino acid sequence, indicating that the mutations of these heading date alleles might not be the cause of the heading date changes. Further expression analysis by qRT-PCR founded that the expression level of heading date alleles DTH8 of WY18 and OsDof12 of WY26 were higher than that of HJX74, indicating that their expression was regulated by the upstream regulatory region. The regulation of this expression might be the reason for the variation of heading date. The WY24 se14 and WY61 Hd1 had obvious sequence deletions, leading to loss function of se14 and Hd1 alleles which probably were the main reason for heading date variations of WY24 and WY61.

Key words: Rice, Heading date, Single segment substitution line, Allelic gene, Genetic differentiation

CLC Number:

S511.03

GAO Ruiyu, ZHANG Hua, XUAN Ning, LIU Xu, ZHANG Hao, ZHANG Mengqi, YAO Fangyin. Variation Analysis of Heading Date Alleles Using Rice Single Segment Substitution Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 29-34. doi: 10.7668/hbnxb.20192258.

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