Study on Cloning and Expression Patterns of Goat C/EBPα, C/EBPβ and C/EBPδ Genes

doi:10.7668/hbnxb.20190869

Abstract

Abstract: This experiment was conducted to study the sequence characteristics, physicochemical properties of goat C/EBPα, C/EBPβ and C/EBPδ genes and proteins, and the expression patterns in goat tissues and in the differentiation of goat intramuscular precursor adipocytes.The C/EBPα, C/EBPβ and C/EBPδ of goat were cloned by RT-PCR method and the biological characteristics were analyzed using the relevant biological software. qPCR was used to detect the expression levels of C/EBPα, C/EBPβ and C/EBPδ mRNA in different tissues and preadipocyte of goat. The results showed that the sequence of C/EBPα was 1 062 bp, C/EBPβ was 1 056 bp and C/EBPδ was 873 bp. The proteins encoded by C/EBPα, C/EBPβ and C/EBPδ were all unstable hydrophilic basic proteins, and had no signal peptide and transmembrane domain. The expression of three genes was detected in different tissues of goats. The expression level of C/EBPα was highest in subcutaneous fat, and the expression pattern of C/EBPβ and C/EBPδ was similar, and the expression level was highest in lung.The expression patterns of C/EBPα and C/EBPδ were similar in the differentiation of adipose precursor cells, and the expression level was the highest at 0 d after differentiation, and the expression level was decreased with the increase of differentiation time. C/EBPβ was found in intramuscular precursor fat. The expression of C/EBPβ in the cells increased with the number of days of induced differentiation, and the expression level was highest at 7 days of differentiation. Based on the above studies, it is speculated that the three genes play an important role in the process of goat adipose differentiation.

Key words: Goat, Gene cloning, Expression, Adipose differentiation

CLC Number:

Q78
S826

CHI Yongdong, WANG Yong, ZHAO Yue, ZHU Jiangjiang, LIN Yaqiu. Study on Cloning and Expression Patterns of Goat C/EBPα, C/EBPβ and C/EBPδ Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 231-238. doi: 10.7668/hbnxb.20190869.

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