Establishment of cDNA-AFLP Analysis System for Castor

doi:10.3969/j.issn.1000-7091.2013.02.016

Abstract

Abstract: In this paper,we studied the factors affecting cDNA-AFLP marked castor differentialgene,estab-lished the suitable cDNA-AFLP analysis system for caster. The results indicated that the concentration of the RNA can be the highest when the lysate volume was 1. 4mL. The enzyme combination EcoR Ⅰ and Mse Ⅰ digested ds-cDNA thoroughly for 6 h. Then it was linked with the connection and the product was used for pre-amplified. The re-producible result and the rich bands can be obtained that the pre-amplified product was diluted 15-fold then was se-lective amplified. The research could provide important evidence for further studying thegene of caster on plant height,leaf type and so on.

Key words: cDNA-AFLP, Castor, Establishment

CLC Number:

S565. 6

WANG Wen-yue, LIguo-rui, HUANG Feng-lan, SHANG Yu-si, WANG Chao, LUO Rui, QIU Jing, WANG Dan, ZHANG Zhi-yong, CHEN Yong-sheng,. Establishment of cDNA-AFLP Analysis System for Castor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 86-90. doi: 10.3969/j.issn.1000-7091.2013.02.016.

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