Cloning and Expression Analysis of S-adenosylmethionine Decarboxylasegene from Lotus corniculatus L．

doi:10.3969/j.issn.1000-7091.2013.02.015

Abstract

Abstract: S-adenosylmethionine decarboxylase(SAMDC;EC 4. 1. 1. 50)is a key rate-limiting enzyme located in the polyamine biosynthesis pathway. According to the sequence information of EST from Lotus corniculatus var. ja-ponicus Regel,a cDNA of SAMDC was cloned from Lotus corniculatus,and named LcSAMDC1. The full-lengthge-nome sequence of LcSAMDC1 was 2 626 bp,including 3 introns. The full-length cDNA sequence of LcSAMDC1 was 1 799 bp,with 3 characteristic ORFs,which were tORF,sORF and mORF,coding 2,55,354 amino acid residues re-spectively. The deducted protein of mORF was with 38. 6 kDa molecular weight,and had two highly conserved function domains(proenzyme cleavage site and PEST domain). Semi-quantitative RT-PCR analysis showed that,the expression of LcSAMDC1 was higher in young leaves and roots than in stems,but failed to be detected in mature leaves.

Key words: Lotus corniculatus L． , Polyamine, S-adenosylmethionine decarboxylase, LcSAMDC1

CLC Number:

LU Yu-lan, SUN Yan-xiang, FENG Xue, ZHAO Xue-liang. Cloning and Expression Analysis of S-adenosylmethionine Decarboxylasegene from Lotus corniculatus L．[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 78-85. doi: 10.3969/j.issn.1000-7091.2013.02.015.

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