SRAP Markers Linked to Powdery Mildew Resistancegene in Melon

doi:10.3969/j.issn.1000-7091.2013.02.014

Abstract

Abstract: Parental lines and(No. 1Shouji×Huanghou)F1,F2and backcross populations were scored for re-sponse to inoculation with Podosphaera xanthiirace 1. The sequence related amplified polymorphism(SRAP)tech-nique and bulk segregant analysis(BSA)were used to identify molecular markers linked to the resistance of melon powdery mildew. The resistance to P. xanthii race 1 in No. 1 Shouji was controlled by one single dominantgene. A total 1 600 pairs SRAP primers could detect the polymorphism between the resistant pool and susceptible pool. An unique 100 bp fragment was amplified with the primer me4em37. It was identified that is linked to resistancegene of No. 1 Shouji at a distance of 17. 0 cM.

Key words: Cucumis melo L．, Powdery mildew, Disease resistancegene, SRAP marker

CLC Number:

ZHANG Xue-jun, JI Juan, LI Mei-hua, WANG Hao-jie, YI Hong-ping,. SRAP Markers Linked to Powdery Mildew Resistancegene in Melon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 73-77. doi: 10.3969/j.issn.1000-7091.2013.02.014.

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