Analysis of the Functions of Three Wheat Powdery Mildew Resistance-related Genes by A Transient Expression

doi:10.3969/j.issn.1000-7091.2012.05.006

Abstract

Abstract: Transient expression system was used to analyze the function of three wheat powdery mildew resistance-related genes,CAF1,BI-1,and DAD1.Target genes were constructed into vectors pAHC25 and transformed into leaf epidermal cells of a powdery mildew-susceptible wheat variety Jingdong 8 by bombardment.GUS gene was co-transformed with target genes to mark the transformed cells.The penetration efficiency(PE) after bombarding of target gene was compared with control,then evaluated the effects of the target gene′s products on the invasion of powdery mildew.The results implied that transient expression of gene CAF1 could partly inhibit the penetration of conidia spores and formation of haustoria(PE was reduced to(37.37±3.2)%,as compared with the control of(54.75±0.6)%),while gene BI-1(PE was(54.31±1.7)%,PE of control was(52.37±1.8)%) and gene DAD1(PE was(57.34±1.1)%,PE of control was(50.93±1.3)%)showed no inhibiting effects.

Key words: Wheat powdery mildew, Transient expression, CAF1 BI-1 DAD1

CLC Number:

S435.121.3

WANG Yu-hui, XIN Ming-ming, SONG Na, PENG Fu-xiang, TANG Hua-shan, LI Ying-hui, NI Zhong-fu, XIE Chao-jie, SUN Qi-xin. Analysis of the Functions of Three Wheat Powdery Mildew Resistance-related Genes by A Transient Expression[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 28-32. doi: 10.3969/j.issn.1000-7091.2012.05.006.

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