Prokaryotic Expression of the Envelope Protein of Goose Flavivirus and Antigenic Analysis

doi:10.3969/j.issn.1000-7091.2012.05.007

Abstract

Abstract: To obtain the recombinant protein used for serologic diagnosis of and as a subunit vaccine against avian flavivirus infection,the envelope(E)gene of goose flavivirus was cloned,expressed and purified.One pair of primers was designed according to the published sequence of complete genome of the goose flavivirus strain JS804(GenBank accession number JF895923).The E gene was amplified by RT-PCR and cloned into the prokaryotic expression vector pET32a(＋) to construct the recombinant plasmid pET32a-E,which was then transformed into Escherichia coli BL21(DE3).The fusion protein was expressed after induced by IPTG and presented mainly in the form of inclusion body.The results of the Western Blot and the IFA test indicated that the expressed protein possessed strong reactinogenicity and immunogenicity.The research made a foundation for further research on the serological diagnosis and the construction and function analysis of E protein,and the E protein can serve as a potential vaccine to prevent avian flavivirus infection.

Key words: Avian flavivirus, E protein, Prokaryotic expression, Antigenicity

CLC Number:

S852.65

HUANG Xin-mei, LI Yin, ZHAO Dong-min, LIU Yu-zhuo, ZHANG Jing-feng, HAN Kai-kai, NIU Hui-min. Prokaryotic Expression of the Envelope Protein of Goose Flavivirus and Antigenic Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 33-37. doi: 10.3969/j.issn.1000-7091.2012.05.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.05.007

http://www.hbnxb.net/EN/Y2012/V27/I5/33

[1]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[2]	WANG Ya'nan, LIANG Yanyan, YAN Wenpei, ZHANG Yinping, LI Qiang, WU Xiuju. Cloning and Prokaryotic Expression Analysis of AhOMT1 Gene from Asarum heterotropoides [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 62-70.
[3]	LIAN Kaiqi, JI Shuang, ZHOU Lingling, SHI Zhiqi, WANG Fei, ZHANG Yuanchen. Cloning and Prokaryotic Expression of a clip-type Serine Protease Gene from Mythimna separate [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 195-201.
[4]	JIA Weizhe, JIAO Bo, WANG Jiao, CHEN Wenye, YANG Fan, LIU Yongwei, DONG Fushuang, ZHAO Liqun, ZHOU Shuo. *Cloning and Expression Analysis of Calmodulin-like Protein TaCML8-A* Gene in Wheat** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 1-8.
[5]	FAN Huifen, SUN Tianjie, SU Weihua, XIAO Fuming, ZHANG Jie, WANG Dongmei. Preparation of GmWRKY50 Protein Antibody and Analysis of Its Protein Level Expression [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 28-34.
[6]	YE Fahui, CAO Dong, ZHAO Caixia, SHEN Jicheng, LIU Ruijuan, LIU Demei, CHEN Wenjie, ZHANG Huaigang. *Effect of Avenin-like b Protein in Aegilops tauschii* Cosson on Flour Quality in Wheat** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 143-147.
[7]	JIANG Nan, ZHENG Qianqian, LI Qianwen, TU Jian, SONG Xiangjun, SHAO Ying, LIU Hongmei, QI Kezong. Prokaryotic Expression Vector Construction,Expression,Purification and Identification of the APEC Virulence Gene ygeG [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 184-190.
[8]	YU Tianfei, XIE Pengyu, SUN Wanshu, LI Jing, YIN Haichang, LI Ming, YU Zhidan. *Prokaryotic Expression of Variable Domain of Monoclonal Antibody Against Goose parvovirus* NS1 Protein** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 211-215.
[9]	XU Huan, DUAN Shengwen, FENG Xiangyuan, ZHENG Ke, YANG Qi, WANG Qiming, CHENG Lifeng, PENG Yuande. High Level Expression of Pectate Lyase Gene from Microbiology for Ramie Degumming and Its Bioinformatics Analysis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 18-23.
[10]	LEI Qidong, SUN Xudong, XU Huini. *Cloning and Prokaryotic Expression of Arabidopsis AtTCP4* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 24-28.
[11]	LI Moxiao, BIAN Zhe, ZHOU Qihui, LIU Yuwei, GONG Xiaodong, GU Shouqin, HAN Jianmin. Analysis of the Gene Structure,Prokaryotic Expression and Expression Pattern of StRTG2 in Setosphaeria turcica [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 191-196.
[12]	WANG Haibo, LI Furong, YANG Jincui, GAO Yong, GUO Junyun. Cloning and Prokaryotic Expression of Phosphoglucomutase Genes cPGM and pPGM from Jatropha curcas [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 23-30.
[13]	LIU Qing, SUN Tingzhao, GAI Shupeng, ZHANG Yuxi, LIU Chunying. Characteristic and Expression Analysis of a Transcription Factor PsZFP1 in Tree Peony [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 67-73.
[14]	LI Jiahao, XIE Minqiu, WAN Chuanyin, ZUO Ruijie, LU Liming, LI Liqin. *Prokaryotic Expression of Transcription Factor StTCP13* in Potato and Its Function of Salt Stress** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 33-39.
[15]	HU Yueqing, WANG Ruozhong, HUANG Zhigang, XIAO Langtao. *Cloning and Prokaryotic Expression of Transcription Factor StWRKY57* Gene in Potato** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 10-17.

Please choose a citation manager

Content to export