Study on StBAG3 Gene Mediated Potato Resistance to Late Blight

doi:10.7668/hbnxb.20190499

Abstract

Abstract: In order to explore the possible functions of StBAG3 gene derived from potato in the process of potato resistance establishment, this study used the in vitro leaves of Shepody as experimental material, and used the transient expression system of potato to explore the preliminary function of StBAG3 gene. The results showed that Phytophthora infestans was inoculated on the leaves of potato expressing StBAG3 gene transiently, after 24, 48, 72, 96 h, the relative lesions area was significantly reduced by 1.92-39.15 percentage points compared with the control, indicating transient expression of the StBAG3 gene significantly increased potato resistance to Phytophthora infestans. This result was also confirmed by the staining of necrotic cells at the inoculation site. The results of qualitative and quantitative determination of H₂O₂ showed that the accumulation of H₂O₂ at the inoculation site after transient expression of StBAG3 gene was not significantly different from 0 to 48 h after inoculation, they significantly different from control at 72,96 h after inoculation, both reached the maximum at 72 h, 0.146, 0.086 μmol/g, respectively. The results of ROS scavenging enzymes activity assay showed that the activity of SOD and CAT enzymes were varied with the inoculation time after transient expression of StBAG3 gene, but both were higher than the control leaves at 0-96 h. POD activity was not significantly different from the control leaves at 0-72 h after inoculation, only significantly higher than control leaves at 96 h after inoculation. It was indirectly proved that H₂O₂ was involved in StBAG3 gene mediated positive regulation establishment process of potato resistance to late blight. The results showed that StBAG3 gene can increase potato resistance to late blight.

Key words: Potato, Transient expression, StBAG3, Potato late blight, Disease resistance

CLC Number:

S432.1

HOU Dingyi, ZHANG Xiaoluo, KANG Liru, ZHAO Jun, ZHANG Zhiwei. Study on StBAG3 Gene Mediated Potato Resistance to Late Blight[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 193-199. doi: 10.7668/hbnxb.20190499.

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