Extraction of High Quality RNA from Whangkeumbae Pear

doi:10.3969/j.issn.1000-7091.2013.02.013

Abstract

Abstract: Isolation of high-quality RNA is the necessary premise ofgene cloning and Northern blot. The Whangkeumbae Pear has the characteristic of the high level of polyphenol,polysaccharides and other secondary me-tabolities. Numerous methods of the predecessors have been taken into consideration,and three of them were chosen to be utilised to compare. The result shows that:modified 3% CTAB method and TIANGEN RNAplant plus Reagent were suitable for total RNA extraction from Whangkeumbae Pear,but TIANGEN RNAplant plus Reagent is more suitable. The electrophoresis of RNA was showed less degradation and higher purity.

Key words: Whangkeumbae Pear, Fruit, RNA extraction method

CLC Number:

S661. 03

SUN Wen, SHI Hai-yan, ZHANG Yu-xing. Extraction of High Quality RNA from Whangkeumbae Pear[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 70-72. doi: 10.3969/j.issn.1000-7091.2013.02.013.

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http://www.hbnxb.net/EN/Y2013/V28/I2/70

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