Gene Expression Related to Sugar Content During Storage of Kiwifruit under Room Temperature with CPPU Treatment

doi:10.7668/hbnxb.201751175

Abstract

Abstract: In order to study the regulation mechanism in the sugar meta bolismin of CPPU treated kiwifruit during the storage,this study analyzed the differences in total soluble solids(TSS), titratable acid(TA) and soluble sugar conten(SSC) between 20 mg/L CPPU-treated and water-treated kiwifruits after two, four, six, or eight days of storage, and differential gene expression related to these compounds using high-throughput sequencing. The study found that, regardless of the treatment or the control, the soluble sugar content gradually increased during the storage, but the soluble sugar content of the CPPU-treated fruit was always lower than that of the control fruit. CPPU treatment inhibited the expression of gene Achn087691 (glucose-6-phosphate isomerase) in starch and sucrose metabolism and Achn203191 (triosephosphate isomeras) in fructose and mannose metabolism, and promoted early expression of gene Achn069851 (hexokinase) in starch and sucrose metabolism, but showed early inhibition,medium-term promotion, late suppression of gene Achn161931 (UDP-glucose 6-dehydrogenase), gene Achn206141 (udp-galactose-4-epimerase), and gene Achn295291 (pectin methylesterase). It was speculated that 20 mg/L CPPU treatment significantly affected the soluble sugar content and sugar metabolism related gene expression in kiwifruit(Actinidia deliciosa Xuxiang), resulting in a decrease in TSS and SSC during storage at room temperature and accelerated the softening of fruit.

Key words: Kiwifruit, Storage, RNA-seq, Sugar content

CLC Number:

S663.03
Q78

GUO Linlin, LUO Jing, PANG Rongli, WANG Ruiping, HUANG Yunan, QIAO Chengkui, LI Jun, PANG Tao, XIE Hanzhong. Gene Expression Related to Sugar Content During Storage of Kiwifruit under Room Temperature with CPPU Treatment[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 40-45. doi: 10.7668/hbnxb.201751175.

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