Changes of Ethylene, Related Enzymes and Their Genes Expression During Softening of Premature Apple Fruits

doi:10.7668/hbnxb.201751544

Abstract

Abstract: The fruit softening of precocious apple cultivar is an important factor for promotion of apple production. It is important to elucidate the mechanism of fruit softening to prolong the fruit storage period and maintain the fruit quality. In this experiment, Mato 1 and XU2-5 were used as the materials to analyze the changes of fruit hardness, ethylene release rate, related enzyme activity and related gene expression during fruit softening. The main results showed that, along with the ripening of fruits, the fruit hardness decreased and the ethylene release rate increased. The ethylene release rate of Mato 1 was significantly higher than that of XU2-5. The relative expression pattern of MdACS6 gene was basically the same in the two varieties, of which the relative expression level was low in early time, and gradually increased after that. With the ripening of fruits, the relative expression amount of MdPG1 gene and the change of enzyme activity in Mato 1 were consistent, which reached the highest at 100 d after flowering, while XU2-5 showed the consistency between relative expression level of MdPG1 gene and the change of enzyme activity in 80-90 d after flowering, of which the relative expression of MdPG1 was the highest at 90 d after flowering, and the enzyme activity was the highest at 100 d after flowering. In 80-85 d after flowering, the relative expression of MdPE gene and PE enzyme activity in the two varieties (lines) presented an increasing trend, and the enzyme activity reached to a peak value. In 90-100 d after flowering, the relative expression of MdPE gene decreased in the two varieties (lines), while the PE enzyme activity increased gradually. The LOX enzyme activity in the two varieties (lines) decreased first and then increased, and the lowest point appeared at 85 d after flowering. The relative expression of MdLOX gene in XU2-5 increased first and then decreased, and the peak appeared at 85 d after flowering, while the relative expression of MdLOX gene in Mato 1 increased gradually, and the highest value appeared at 100 d after flowering.In summary,taking measures to regulate gene expression may be effective in regulating fruit softening.

Key words: Apple, Precocious cultivar, Fruit softening, Ethylene, Gene expression, Enzymes

CLC Number:

S661.01

LI Shijun, YIN Baoying, LI Jia, LI Zhongyong, ZHANG Xueying, XU Jizhong. Changes of Ethylene, Related Enzymes and Their Genes Expression During Softening of Premature Apple Fruits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 104-109. doi: 10.7668/hbnxb.201751544.

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