Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (3): 175-185. doi: 10.7668/hbnxb.20192879

Special Issue: Apple Soil fertilizer Plant protection Hot Article

• Resources & Environment · Plant Protection • Previous Articles     Next Articles

Effects of Pyroligneous Acids on the Growth and Main Characteristics of Replanted Soil of Malus angustifolia

LU Xiaoyue1,2, WANG Ziye1,2, HAN Jianwei1,2, XU Lu1,2, ZHANG Xiaofei1,2, HAN Jie1,2, WANG Zhigang1,2, LIU Yue1,2, SUO Xiangmin3, , YAN Aihua1,4,   

  1. 1. College of Forestry,Hebei Agricultural University,Baoding 071000,China
    2. Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei,Baoding 071000,China
    3. Shijiazhuang Institute of Fruit Trees,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050061,China
    4. Hebei City Forest Health Technology Innovation Center,Baoding 071000,China
  • Received:2022-02-26 Published:2022-06-30
  • Contact: SUO Xiangmin, YAN Aihua


In order to clarify the mechanism of pyroligneous acids improving replantation soil,the effect of pyroligneous acids treatment on plant growth was studied by field experiment using apple rootstock-Malus micromalus as experimental material.The main physical and chemical properties and enzyme activities of soil in July and October were analyzed based on Illumina high-throughput sequencing to analyze the changes of microbial diversity in rhizosphere soil.The results showed that compared with the control,the annual increase of plant height,ground diameter and leaf area of Malus angustifolia seedlings were significantly increased after 100-fold pyroligneous acids irrigation.Pyroligneous acids root irrigation treatment significantly increased the contents of main nutrients and enzyme activities in the soil of replanting disease.The contents of soil organic matter,total nitrogen,alkali hydrolyzable nitrogen,available phosphorus and available potassium were 1.31,1.38,1.20,1.60 and 1.65 times of CK1,respectively in July,and 1.12,1.03,1.58,1.40 and 1.25 times of CK2 in October,respectively.Sucrase and urease activities increased significantly in July and October.Pyroligneous acids increased the microbial diversity of rhizosphere soil in summer and autumn.Under CK and pyroligneous acids treatments in July and October,the top five dominant gates of rhizosphere bacteria were Proteobacteria,Acidsobacteria,Gemmatimonadetes,Chloroflexi and Rokubacteria;at the genus level,they were mainly uncultured_bacterium_c_Subgroup_6, uncultured_bacterium_f_Gemmatimonadaceae, uncultured_bacterium_o_Rokubacteriales, RB41 and MND1. The top five dominant gates of rhizosphere fungi were Ascomycota,Basidiomycota, Mortierellomycota,Chytridiomycota and Glomeromycota.At the genus level,they were mainly Cladosporium,Mortierella,Ilyonectria,Guehomyces and Fusarium.According to the correlation network map,the beneficial bacteria RB41 and uncultured_bacterium_f_Gemmatimonadaceae in the rhizosphere and the pathogenic fungi Fusarium and Ilyonectria were negatively correlated with other populations.Pyroligneous acids root irrigation can increase soil nutrients and enzyme activity,increase soil microbial diversity,improve soil microbial community structure,promote plant growth,enhance plant resistance and reduce the harm of replanting disease.

Key words: Pyroligneous acids, Apple replant disease, Soil microbes, Growth indicators

Cite this article

LU Xiaoyue, WANG Ziye, HAN Jianwei, XU Lu, ZHANG Xiaofei, HAN Jie, WANG Zhigang, LIU Yue, SUO Xiangmin, YAN Aihua. Effects of Pyroligneous Acids on the Growth and Main Characteristics of Replanted Soil of Malus angustifolia[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 175-185. doi: 10.7668/hbnxb.20192879.

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