Abstract: Study unknown DOFH10 (DNA binding with one finger) genes in peanuts,cloning of DOFH10 genes with PCR methods according to cDNA of DOF transcription factor GW391728 with primers s162-a1469 and primers s30-a1469 from 16 different peanut cultivars of Arachis hypogaea L.,sequences comparison,analysis on enzyme site and 3D structure and Western Blot on DOFH10 proteins were conducted. Two types of DOFH10 genes were separated from genome DNA of 5 cultivars with primers s162-a1469,type A DOFH10 was only existed in four cultivars with multiple seeds per fruit and one cultivar with two seeds per fruit. They were identical to XM_016100960.2 from Arachis duranensis chromosome A03 and to XM_016334585.2 from Arachis ipaensis chromosome B03 respectively. With primers s30-a1469,only type B DOFH10 was cloned from all the 16 cultivars used including all the six cultivars with multiple seeds per fruit. Type B DOFH10 had identical RNA and protein sequences at DOF zinc finger region to that of putitive clathrin assembly protein XP_016199412.2 encoded by gene on chromosome B01 of Arachis ipaensis. Multiple possible myristoylation sites scattered on DOFH10 protein,both results suggested that transportation of DOFH10 was probable via vesicle membrane traffic related to clathrin. DOFH10 protein was specifically expressed in developing cotyledons displayed by Western Blot. A and B two types of DOFH10 genes existed in cultivated peanut. The type B was present in all 16 peanut cultivars,and was an essential gene,was possibly transported into nucleus through clathrin dependant vesicle pathway. The type A DOFH10 tended more appeared in cultivars with multiple seeds per fruit,probably related to cell division in early fruit development.

Key words: DOF, Transcription factor, Peanut, Clathrin related, Fruit development

CLC Number: 

• Q78