Genetic Diversity and Population Structure Analysis of Zanthoxylum Germplasms by SRAP

doi:10.7668/hbnxb.2016.05.018

Abstract

Abstract: To explore the genetic diversity and population structure of Zanthoxylum germplasm,the genetic diversity among 269 accessions of Zanthoxylum which collected from Shaanxi,Shanxi,Yunnan,Sichuan and Gansu Provinces was studied by SRAP Markers.The results showed that 16 pairs of SRAP primes,amplified clear repetitive and intense bands,were selected from 120 combinations.A total of 169 clear bands were amplified,there were 151 polymorphic bands and the percentage of polymorphic bands was 93.8%;The UPGMA clustering showed that the populations were divided into two main groups, Z.armatum DC.and Z.bungeanum Maxim.The highest genetic diversity level was observed within Shanxi Province,the minimum genetic distance was between Shaanxi and Gansu,the maximum was between Shaanxi and Yunnan.The AMOVA showed that the genetic variation was mainly caused by variation of intra-provenances(74%),rather than among provinces(26%);the results of population genetic structure were consistent with the UPGMA clustering and indicated obvious gene flow among provinces.The results provide a basis for the collection,classification and identification of Zanthoxylum resources.

Key words: Zanthoxylum germplasms, SRAP Markers, Genetic diversity, Genetic structure

CLC Number:

S573.03

LI Lixin, YANG Tuxi, WEI Anzhi, FENG Shijing, CHEN Lü, HOU Na. Genetic Diversity and Population Structure Analysis of Zanthoxylum Germplasms by SRAP[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 122-128. doi: 10.7668/hbnxb.2016.05.018.

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