Analysis of EMS Treatment Conditions on Castor and Mutants are Detected by Molecular Marker

doi:10.7668/hbnxb.2015.04.009

Abstract

Abstract: In order to innovate castor germplasm,hulled castor seeds were treated with EMS at 6 levels (0,0.02,0.03,0.04,0.05,0.06 mol/L)and different time(4,8,12 h),The results showed that lethal concentration 50 was at 0.04 mol/L and 4 h,the average taproot length was 10.45 mm,and the concentration of EMS reached to 0.06 mol/L,the seeds could not germinate completely.With the increasing of treatment time and concentration,castor seed germination rate and average relative root length showed a downward trend;ISSR molecular marker analysis showed that DNA variation existed in M₂ by EMS.The primer P91 detected 13 variation loci.

Key words: Castor, EMS, Median lethal dose, Molecular marker

CLC Number:

S565.603

LIU Peng, LI Xu-xin, ZHANG Chun-lan, ZHANG Yuan-yuan, ZHANG Wei-guo. Analysis of EMS Treatment Conditions on Castor and Mutants are Detected by Molecular Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 47-50. doi: 10.7668/hbnxb.2015.04.009.

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