Cultivation of Herbicide Resistance Rice with Low Amylose by Molecular Marker-assisted Selection

doi:10.7668/hbnxb.20190894

Abstract

Abstract: In order to improve the taste quality and production practices of rice,a new rice line with both low amylose content and imidazolinone herbicide resistance was developed. Nanjing 9108 which with low amylose content was used as gene donor to cross with the sterile single plant selected from the dominant male sterile isolation population. Nanjing 9108 was used twice to backcross with the hybrid products to construct BC₂F₁ population. Tetra-primer ARMS-PCR was used to analyze BC₂F₁ population taking advantage of the SNP(Single Nucleotide Polymorphism) in Wx-mq gene. It could be accurately distinguished the Wx-mq homozygous genotype, Wx-mq heterozygous genotype and Wx-mq homozygous genotype according to the PCR results. The sterile and fertile single plants with low amylose content were screened out. Then Jinjing 818 with Acetolactate Synthase gene(ALS) was used as the donor parent of herbicide resistance gene to cross with the selected sterile single plant with low amylose content. The allele-specific PCR (AS-PCR) was used to amplify F₁ according to the SNP in the coding regions of ALS gene to screen out herbicide resistance homozygous genotype,susceptible herbicide homozygous genotype and herbicide resistance heterozygous genotype. The results of ARMS-PCR identification showed that 172 single plant band patterns detected in the BC₂F₁ sterile population were consistent with those of Nanjing 9108,containing waxy genes and waxy traits;160 individual plants didn't contain glutinous genes; 468 individual plants had a heterozygous genotype for parents.The herbicide resistance was identified by AS-PCR. The results showed that among 1 200 individual plants of F₁ population,868 individual herbicide resistant plants and 332 individual herbicide sensitive plants were detected. There were 624 homozygous genotypes and 576 heterozygous genotypes. The 6 new rice lines with low amylose content and herbicide resistance were gained in F₅ generation after the selection using the molecular marker with low amylose content and herbicide resistance.

Key words: Rice, Acetolactate synthase, Tetra-primer ARMS-PCR, Wx-mq, Molecular marker, Low amylose

CLC Number:

S511.03

ZHANG Hao, LIU Xu, XUAN Ning, ZHANG Hua, GAO Ruiyu, ZHAO Qianqian, YAO Fangyin. Cultivation of Herbicide Resistance Rice with Low Amylose by Molecular Marker-assisted Selection[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 78-84. doi: 10.7668/hbnxb.20190894.

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