Proteomics Analysis of Anti-senescence Rice Leaf under Nutrient Stress Condition During Late Development Stage

doi:10.7668/hbnxb.2015.04.010

Abstract

Abstract: In order to search for the effects and the mechanism of nutrient stress on anti-senescence rice,the proteins expression profile of functional leaves at the booting stage of hybrid rice Longping 001 was studied by using the approach of plant proteomics.Proteins collected from Longping 001 leaves were separated with 2-DE and then analyzed with Imagemaster 2D Elite 5.0.Twenty-six differentially expressed proteins were obtained,among which 17 proteins were identified by ESI-Q MS/MS.The functions of these 17 proteins were analyzed and the results showed that:hybrid rice Longping 001 has strong adaptability to nutrient stress.The expression abundance of photosynthesis,nutrient-reused and stress resistance associated proteins increased in leaves,which should be beneficial to produce more material for plant development and protect photosynthetic organism away from senescence.

Key words: Rice, Anti-senescence, Nutrient stress, Proteomics

CLC Number:

SHAO Cai-hong, WANG Ping, CHEN Jin, LI Yao, LIU Guang-rong, XIE Jin-shui, PENG Chun-rui. Proteomics Analysis of Anti-senescence Rice Leaf under Nutrient Stress Condition During Late Development Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 51-58. doi: 10.7668/hbnxb.2015.04.010.

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References

[1] 金松恒,蒋德安,王品美,等.水稻孕穗期不同叶位叶片的气体交换与叶绿素荧光特性[J].中国水稻科学,2004,18(5):443-448.
[2] Lin A,Wang Y,Tang J,et al.Nitric oxide and protein S-nitrosylation are integral to Hydrogen peroxide-induced leaf cell death in rice[J].Plant Physiology,2012,158(1):451-464.
[3] Shi B,Ni L,Zhang A,et al.OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice[J].Molecular Plant,2012,5(6):1359-1374.
[4] Jiao B B,Wang J J,Zhu X D,et al.A novel protein RLS1 with NB-ARM domains is involved in chloroplast degradation during leaf senescence in rice[J].Molecular Plant,2012,5(1):205-217.
[5] Qiao Y,Jiang W,Lee J,et al.SPL28 encodes a clathrin-associated adaptor protein complex 1,medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa)[J].The New Phytologist,2010,185(1):258-274.
[6] Dai X B,Cao S Q,Xu X M,et al.Study on a mutant with low content chlorophyll b in a high yielding rice and its photosynthesis properties[J].Acta Botanica Sinica,2000,42(12):1289-1294.
[7] 许晓明,张荣铣,唐运来.低叶绿素含量对突变体水稻吸收光能分配特性的影响[J].中国农业科学,2004,37(3):339-343.
[8] 王丹英,章秀福,邵国胜,等.不同叶色水稻叶片的衰老及对光强的响应[J].中国水稻科学,2008,22(1):77-81.
[9] 董桂春,王熠,于小凤,等.不同生育期水稻品种氮素吸收利用的差异[J].中国农业科学,2011,44(22):4570-4582.
[10] 王为木,杨肖娥,李华,等.低钾胁迫对两个耐钾能力不同水稻品种养分吸收和分配的影响[J].中国水稻科学,2003,17(1):52-56.
[11] 谢金水,邵彩虹,唐秀英,等.养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋白质组学分析[J].中国水稻科学,2011,25(2):143-149.
[12] 邵彩虹,钱银飞,唐秀英,等.养分胁迫对水稻籽粒灌浆充实影响的蛋白质组学研究[J].中国水稻科学,2012,26(3):267-274.
[13] 王经源,陈舒奕,梁义元,等.ISO-DALT双向电泳方法的优化与改进[J].福建农林大学学报:自然科学版,2006,35(2):187-190.
[14] 夏其昌,曾嵘.蛋白质化学与蛋白质组学[M].北京:科学出版社,2004:278.
[15] 李瑞,周玮,李丽,等.水稻叶片自然衰老过程中Rubisco大亚基的含量变化[J].中国水稻科学,2009,23(5):555-558.
[16] 彭新湘,彭少兵.水稻叶片在自然衰老过程中1,5-二磷酸核酮糖羧化酶/加氧酶的降解(英文)[J].植物生理学报,2000,26(1):47-53.
[17] 陈宗良,罗喜娜,杨特武,等.不同施肥水平对水稻生育进程中功能叶光合性能及水势变化的影响[J].华中农业大学学报,2007,26(6):
[18] 王忠.植物生理学[M].北京:中国农业出版社,2002:126.
[19] 罗赟星,赵辅昆,罗贵民,等.S-腺苷甲硫氨酸的细胞生物化学功能及其开发应用研究[J].工业微生物,2006,36(1):54-59.
[20] 李昌澎,周琳璘,陈亮,等.S-腺苷甲硫氨酸代谢途径相关基因在小麦水分胁迫中的表达[J].西北植物学报,2011,31(6):1120-1126.
[21] 孔垂华,胡飞,谢华亮,等.外源多胺对水稻萌发和前期生长的作用及其在土壤中的滞留[J].应用生态学报,1996,7(4):377-380.
[22] 林文雄,吴杏春,梁康迳,等.UV-B辐射增强对水稻多胺代谢及内源激素含量的影响[J].应用生态学报,2002,13(7):807-813.
[23] 施木田,陈如凯.锌硼营养对苦瓜产量品质与叶片多胺、激素及衰老的影响[J].应用生态学报,2004,15(1):77-80.
[24] Moon H,Baek D,Lee B,et al.Soybean ascorbate peroxidase suppresses Bax-induced apoptosis in yeast by inhibiting oxygen radical generation[J].Biochemical and Biophysical Research Communications,2002,290(1):457-462.
[25] 付国良,黄晓红.甘油醛-3-磷酸脱氢酶功能的研究进展[J].生物物理学报,2013,29(3):181-191.
[26] 石之光,叶磊,巩鹏涛,等.乙醇脱氢酶(ADH)家族生物信息学分析[J].基因组学与应用生物学,2009,28(3):429-432.
[27] Klaus W,Wieczorek B H.The V-type H + ATPase:molecular structure and function,physiological roles and regulation[J].Journal of Experimental Biology,2006,209:577-589.
[28] Neumann G,Massonneau A,Martinoia E,et al.Physiological adaptations to phosphorus defficiency during proteoid root development in white lupin[J].Planta,1999,208:373-382.
[29] Wasaki J,Omura M,Osaki M,et al.Structure of a cDNA for an acid phosphatase from phosphate-deficient lupin (Lupinus albus L.) Roots[J].Soil Science and Plant Nutrition,1999,45(2):439-449.
[30] Haran S,Logendra S,Seskar M,et al.Characterization of Arabidopsis acid phosphatase promoter and regulation of acid phosphatase expression[J].Plant Physiology,2000,124(2):615-626.
[31] Gaume A,Mächler F,De León C,et al.Low-P tolerance by maize (Zea mays L.) genotypes:Significance of root growth,and organic acids and acid phosphatase root exudation[J].Plant and Soil,2001,228(2):253-264.
[32] Yan X,Liao H,Trull M C,et al.Induction of a major leaf acid phosphatase does not confer adaptation to low Phosphorus availability in common bean[J].Plant Physiology,2001,125(4):1901-1911.
[33] 张海伟,黄宇,叶祥盛,等.低磷胁迫下甘蓝型油菜酸性磷酸酶对磷效率的贡献分析[J].中国科学:生命科学,2010,40(5):418-427.
[34] 徐晨,凌风楼,徐克章,等.盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J].中国水稻科学,2013,27(3):280-286.
[35] 彭海欢,翁晓燕,徐红霞,等.缺钾胁迫对水稻光合特性及光合防御机制的影响[J].中国水稻科学,2006,20(6):621-625.
[36] 赵明辉,孙建,王嘉宇,等.全基因组分析低氮胁迫下水稻剑叶光合相关基因表达变化[J].中国农业科学,2011,44(1):1-8.
[37] Munns R,Tester M.Mechanisms of salinity tolerance[J].Plant Biology,2008,59:651-681.
[38] 余涛,支立峰,彭论,等.烟草中一条新的S-腺苷甲硫氨酸合成酶基因的克隆及表达分析[J].武汉植物学研究,2004,22(4):277-283.
[39] 陈锐,陈亮,王士强,等.水分胁迫下小麦S-腺苷甲硫氨酸合成酶基因的半定量表达模式分析[J].麦类作物学报,2009,29(6):954-958.
[40] Sparla F,Zaffagnini M,Wedel N,et al.Regulation of photosynthetic GAPDH dissected by mutants[J].Plant Physiology,2005,138(4):2210-2219.
[41] Duff S M G,Sarath G,Plaxton W C.The role of acid phosphatases in plant phosphorus metabolism[J].Plant Physiology,1994,90:791-800.

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