Selection and Identification of the EMS-Induced Drought-resistant Wheat Mutants from Jimai 418

doi:10.7668/hbnxb.20190802

Abstract

Abstract: To obtain specific genetic materials for the study of gene function and genetic improvement of wheat,ethyl methyl sulfonate (EMS) chemical mutagenesis was used to treat the drought-resistant,high-yield,and multi-resistant wheat variety Jimai 418. The field agronomic traits and biological characters were observed and investigated in the M₂. 416 mutant spike lines were selected from 6 961 spike lines,with a mutation frequency of 5.98%.Among them,11 lines were seedlings habit mutants,with a mutation frequency of 0.16%; 12 lines were tiller mutants,with a mutation frequency of 0.17%; 34 lines were leaf mutants,with a mutation frequency of 0.48%; there were 68 plant height mutants with a mutation frequency of 0.97%,62 spike mutants with a mutation frequency of 0.89%,and 132 sterility mutants with a mutation frequency of 1.90%,15 root mutants (0.26%) and 82 other types of mutants. Through the verification of biological and agronomic characters,84 lines with stable inheritance were obtained in M₃,including 43 stem mutants,23 spike mutants,7 leaf mutants,4 early-maturing mutants,2 late-maturing mutants,2 awn mutants,2 root mutants and 1 sterile mutant. The analysis of agronomic characters showed that the mutant library was rich in variation types,and the mutation frequencies of plant height,growth period and 1000-grain weight were relatively high. These new mutants provided new materials for the study of wheat functional genome and wheat genetic improvement.

Key words: Wheat, Jimai 418, Mutant, Selection, Identification, Ethyl methane sulfonate (EMS)

CLC Number:

S512.03

Lü Liangjie, CHEN Xiyong, ZHANG Wenying, ZHAO Aiju, SUN Lijing, ZHANG Yingjun, LIU Yuping, WANG Limei, LI Ziqian, LI Hui. Selection and Identification of the EMS-Induced Drought-resistant Wheat Mutants from Jimai 418[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 47-55. doi: 10.7668/hbnxb.20190802.

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