Differential Expression of Ethylene-Induced Genes for Internode Abbreviation of Maize Analyzed with cDNA-AFLP

doi:10.7668/hbnxb.2014.03.006

Abstract

Abstract: To investigate the molecular mechanisms of ethephon-induced stalk internodes shorten in maize, using maize variety Zhengdan 958, the plant leaves were treated with spraying ethephon(ETH) at 225 mL/ha at early elongation stage, and spraying water as a control.Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-AFLP technique.The results showed that 1 635 fragments of expressed genes were obtained with 70 pairs of primers.The expressions of 600 genes were up-regulated(36.7% of the total bands), 564 genes down-regulated(34.5% of the total bands) and the same expressions of 471 genes(28.8% of the total bands) by ethephon induction.Through the BlastX analysis of 30 different gene fragments of TDFs can be divided into six categories by functional analysis, including signal transduction-related genes(accounting for 6.7%), resistance-related genes(accounting for 16.7%), energy and metabolism-related genes(accounting for 20.0%), transcription factor-related genes(accounting for 10%), unknown functional proteins(accounting for 13.3%) and unknown genes(accounting for 33.3%).Ethephon influence the growth of maize through regulating the glutathione S-transferase, aspartic acid protein kinase and auxin-induced protein gene expressions.

Key words: Ethephon, Maize, cDNA-AFLP, Differential expression

CLC Number:

S513.03

HUO Xiu-ai, YANG Bing-yan, LIU Yun-ting, DUAN Hui-jun. Differential Expression of Ethylene-Induced Genes for Internode Abbreviation of Maize Analyzed with cDNA-AFLP[J]. journal1, 2014, 29(3): 27-31. doi: 10.7668/hbnxb.2014.03.006.

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