Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress

doi:10.7668/hbnxb.20190904

Abstract

Abstract: In order to study the role of RcCNGC2 in the salt tolerance of castor,we cloned and obtained the CDS of RcCNGC2,which was a cyclic nucleotide gated ion channel,from the leaves of Tongbi 5. The CDS of RcCNGC2 was analyzed by bioinformatics,and a multiple expression vector was constructed. The results showed that the CDS length of RcCNGC2 was 2 148 bp,encoding 715 amino acids;the molecular weight of the protein was 34.15 ku; the isoelectric point was 9.96; and there were five transmembrane domains. Amino acid sequence analysis showed that RcCNGC2 had the highest homology with Hevea,up to 89.15%. The results of qRT-PCR showed that the expression of RcCNGC2 was significant difference up-regulated in roots(P<0.05),but decreased significantly in the stem and leaf with the increase of NaCl treatment time. Comprehensively, RcCNGC2 played an important signal transduction role in castor under salt stress.

Key words: Ricinus communis L., Cyclic nucleotide gated channel, Expression analysis, Gene cloning, Bioinformatics

CLC Number:

Q786

DUAN Qiong, WANG Xiaoyu, HUO Hongyan, HE Zhibiao, ZHANG Lixue, LIU Xuming, ZHANG Hongyu, MENG Di, ZHANG Jixing. Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 79-86. doi: 10.7668/hbnxb.20190904.

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