Abstract: In order to understand the genetic diversity of synthetic hexaploid wheats,100 ISSR primers were used to detect the genetic diversity among 11 synthetic hexaploid wheats and three common wheat line (TP,HTS-1 and CS).The results indicated that 42 ISSR primers can be amplified clear bands,24 out of 42 primers had stronger ability to discriminate synthetic hexaploid wheats materials.All the 42 ISSR primers amplified 273 bands,per primer ranged from 3 to 12,with an average of 6.5.Among which 170 (62.3%) allelic variations were detected,the number of allelic variations per primer ranged from 1 to 11,with an average of 4.5.The value of allelic polymorphism information content (PIC) ranged from 0.499 to 0.879,on the average of 0.767 per primer,most of them over 0.800.The value of genetic similarity (GS) indexes of 14 materials based on the ISSR data varied from 0.663 to 0.934,with an average of 0.790.Cluster analysis showed that all of them in this study could be clustered into three groups,second group of which are more than any others.Cluster analysis revealed that genetic similarity of 14 materials were high,which suggested that the 14 materials had a near relationship.But overall the genetic diversity in the synthetic hexaploid wheats was relatively large.The cluster analysis also reflected the difference of pedigree and parental origin.All of these indicated that ISSR molecular marker technology can also be used to identification of breeds,evaluation of genetic diversity and construction of genetic maps in wheats.

Key words: Synthetic hexaploid wheats, Genetic diversity, ISSR, Cluster analysis

CLC Number: 

• S512.03