Acta Agriculturae Boreali-Sinica ›› 2018, Vol. 33 ›› Issue (4): 120-125. doi: 10.7668/hbnxb.2018.04.017

Special Issue: Oil crops

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Genetic Diversity Analysis of Main Parents for Hybrid Soybean Based on SSR Markers

BAI Zhiyuan1, ZHANG Ruijun1, WU Guoping2, LIAN Shichao1, YANG Yuhua1, WEI Baoguo1   

  1. 1. Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China;
    2. Gardening Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Received:2018-04-01 Published:2018-08-28

Abstract: To determine the genetic diversity and genetic relationship of the main parents of hybrid soybean in Shanxi Province, 64 pairs of SSR primers were used to analyze the genetic diversity of 32 hybrid soybean parents including 15 maintainer lines and 17 restorer lines. Totally 357 alleles were revealed among 32 parents, 2-12 alleles could be detected at different SSR locus, with the average of 5.578 alleles per SSR marker, of which 322 alleles were detected in the maintainer line, ranging from 2 to 9 with an average of 5.031, and the detection of 320 alleles in the restorer ranged from 2 to 12 with an average of 5. The polymorphism information content (PIC)of the total polymorphism was 0.368-0.900, with an average of 0.666, of which, the PIC variation of maintainer lines ranged from 0.370 to 0.868 with an average of 0.661, and the PIC variation of restorer lines ranged from 0.329 to 0.904 with an average of 0.630. Cluster analysis result showed that the average genetic similarity coefficient (GS)between maintainer lines was 0.637. At the genetic similarity coefficient (GS)of 0.61, the maintainer lines could be grouped into two major groups. At the similarity coefficient of 0.65, the second group could be divided into three subcategories. The average genetic similarity coefficient (GS)between restorer lines was 0.669. At the genetic similarity coefficient (GS)of 0.65, the restorer lines could be grouped into two major groups. The clustering results were related to the geographical origin of cultivars.

Key words: Soybean, Genetic diversity, SSR marker

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Cite this article

BAI Zhiyuan, ZHANG Ruijun, WU Guoping, LIAN Shichao, YANG Yuhua, WEI Baoguo. Genetic Diversity Analysis of Main Parents for Hybrid Soybean Based on SSR Markers[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 120-125. doi: 10.7668/hbnxb.2018.04.017.

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