Allelic Variation of Starch Synthesis Related Genes and Population Structure in Japonica Rice Cultivars

doi:10.7668/hbnxb.2018.06.016

Abstract

Abstract: To evaluate the allelic variation of starch synthesis related genes in Japonica rice cultivars, their population structure and genetic relationship, 34 molecular markers of starch synthesis related genes were used to detect the genetic diversity of 181 Japonica rice cultivars and their population structure and genetic relationship were analyzed combine with 185 SSR/Indel markers. A total of 74 alleles were detected by 34 molecular markers of starch synthesis related genes, ranging from 2 to 4, with an average value of 2.176 alleles per marker. Genetic diversity index ranged from 0.011 to 0.494 with an average value of 0.148. The polymorphism information content (PIC) ranged from 0.011 to 0.399 with an average value of 0.128. Six moderately polymorphic loci (0.25 < PIC < 0.50) and zero highly polymorphic locus (PIC>0.50) were detected, indicating that the genetic diversity of starch synthesis related genes in Japonica rice cultivars was not enough at present. Population structure analysis and UPGMA cluster analysis based on Nei's genetic distance divided the populations into three main groups (POP1-POP3) and one mixed group (Mixed). Most of the cultivars derived from the neighboring regions were clustered into one large group. It could provide a basis for genetic improvement of eating quality traits of rice, and lay a foundation for the subsequent association analysis of starch synthesis-related genes and starch quality traits.

Key words: Japonica rice, Genetic diversity, Population structure, Starch synthesis related genes

CLC Number:

S511.03
Q78

ZHANG Shanlei, YUE Hongliang, ZHAO Chunfang, CHEN Tao, ZHANG Yadong, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, WANG Cailin. Allelic Variation of Starch Synthesis Related Genes and Population Structure in Japonica Rice Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 116-122. doi: 10.7668/hbnxb.2018.06.016.

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