Abstract: The stress response and its physiological and relative gene characteristics of grape under the weak light conditions remains unclearly due to the lacking of research. The plant cell membrane system and metabolic process is also affected by low-light environment. As studies on response to weak light stress could provide for prevention of weak light adversity. Low light stress on one year Yinhong grape as the tested material with pot experiment, which was used to examine their growth changes, protective enzymes, osmotic adjusting materials, gene expression of MYC2 and TRX used Q-PCR under the different levels. Ranging from shading intensity of 0 to 85%(0, 25%, 40%, 70%, 85%).The results showed as follows:the growth and enzyme activities indicated that they all good at shading of T1, while the plant growth was gradually constrained with the enhanced weak light stress, SOD, POD, CAT, soluble protein activity, MYC2 and TRX gene expression increased firstly and then decreased gradually. At the same time, the activity of PRO and MDA was positively related with the degree of shade. The physiological indexes under higher intensity of shading (85%) were decreased significantly, which indicating that severe stress could cause irreversible damage by mediating itself and not adapt the weak light stress by regulating the cell protective enzymes and synthesizing the related genes. The conservation, the breeding of weak light varieties of grape was also discussed on the basis of the research results, it was also believed that more genes related to the weak light response need to be verified in the future to confirm whether they were induced by weak light.

Key words: Yinhong grape, Weak light stress, MYC2 protein, Thioredoxin h-type

CLC Number: 

• Q78

• S663.03