Genetic Diversity Analyzed by EST-SSR Marker and Polymorphic Traits of 31 Paeonia lactiflora Cultivars

doi:10.7668/hbnxb.2016.S1.016

Abstract

Abstract: Genetic diversity of 31 Paeonia lactiflora cultivars was analyzed by EST-SSR marker and phenotypic traits.Eighty EST-SSR primers from Peony were screened against genomic DNA of Xueshanjinhui,Zhencilian,Biandihong and Zitanshengyan,and 52 primer pairs showed the polymorphism,accounting for 65% of total primers.Then the 52 EST-SSR primers showing polymorphism were subjected to reveal the genetic relationships among the 31 Paeonia lactiflora cultivars.The results showed that among the 31 Paeonia lactiflora cultivars,52 EST-SSR primers pairs had 265.2 polymorphic loci,and 5.1 polymorphic loci were detected by each primers pairs.The Nei's distance between cultivars was calculated for cluster analysis.Moreover,the genetic distance based on the agronomic characters,such as flower color,flower form,plant height,plant width,flower diameter,leaf length,blade width,plant type and leaf type was calculated by Euclidean distance.The classification of Paeonia lactiflora cultivars using the Unweighted Pair-group Method Arithmetic Averages (UPGMA) based on the pair distance is mostly consistent with known pedigree information.The 31 Paeonia lactiflora cultivars have been mainly classified into six groups. GroupⅠconsists of Qihualushuang, Zhushapan, Cibai, Yanzhidianhong, Yulinhong, Yinlonghuanzhu, Moziling, Ziyunu,Baiyubing and Ziliantuojin; Group Ⅱ consists of Hongyanfeishuang, Tianshanhongxing, Zitanshengyan and Xueshanziyu; Group Ⅲ consists of Huahonglou and Zifengyu; Group Ⅳ consists of Zhencilian, Pantuoronghua, Baiyupan and Fenchidicui; Group Ⅴ consists of Biandihong, Tuopanxianbao, Dongfanghong, Ziyanfeishuang, Meiguipiaoxiang and Xueshanjinhui; Group Ⅵ consists of Zihongkui, Hongjuhua, Hongpantuojin and Duoyezi. Mantel test revealed that the goodness of fit between EST-SSR marker and phenotypic analyses was significant (r=0.802 3,t=10.324) in analyzing detecting genetic diversity of the cultivars.This study provided theoretic base for new cultivar breeding and industrialization of Paeonia lactiflora in the future.

Key words: Paeonia lactiflora, Molecular marker, Agronomic characters, Genetic diversity, Cluster analysis

CLC Number:

Q78
R282.12

CAI Jian, ZHU Xuan, XIE Shilong, FAN Haiyan, XING Bin, MA Tongfu. Genetic Diversity Analyzed by EST-SSR Marker and Polymorphic Traits of 31 Paeonia lactiflora Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 92-96. doi: 10.7668/hbnxb.2016.S1.016.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.S1.016

http://www.hbnxb.net/EN/Y2016/V31/IS1/92

References

[1] 秦魁杰,李嘉钰.芍药[M].上海:上海科学技术出版社,2000.
[2] 查良平,杨俊,彭华胜,等.四大产地白芍的种质调查[J].中药材,2011,33(7):1037-1040.
[3] 蔡健,谈玉华,马同富.皖西北芍药产业现状的调查[J].园艺与种苗,2015(1):38-40.
[4] 周红涛,胡世林,郭宝林,等.芍药野生与栽培群体的遗传变异研究[J].药学学报,2002,37(5):383-388.
[5] 郭先锋,王莲英.部分芍药种质资源的RAPD分析[J].园艺学报,2007,34(5):1321-1326.
[6] 胡文清,鲁衡,刘伟,等.芍药野生居群父系分析与遗传结构研究[J].园艺学报,2011,38(3):503-511.
[7] 杨柳慧,于晓南.基于表型性状的芍药不同品种群亲缘关系分析[J].植物遗传资源学报,2016,17(2):209-216.
[8] Litt M,Luty J.A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene[J].American Journal of Human Genetics,1989,44(3):397-401.
[9] Jia X P,Shi Y S,Song Y C,et al.Development of EST-SSR in foxtail millet (Setaria italica)[J].Genetic Resources and Crop Evolution,2007,54(2):233-236.
[10] Yao L H,Zheng X Y,Cai D Y,et al.Exploitation of malus EST-SSRs and the utility in evaluation of genetic diversity in malus and pyrus[J].Genetic Resources and Crop Evolution,2010,57(6):841-851.
[11] Murray M G,Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8(19):4321-4325.
[12] 张艳丽,王雁,李正红,等.基于牡丹EST信息的滇牡丹SSR标记开发[J].林业科学研究,2011,24(2):171-175.
[13] 侯小改,王娟,郭大龙,等.牡丹EST资源的SSR信息分析[J].湖南农业大学学报:自然科学版,2011,37(2):172-176.
[14] Panaud O,Chen X,Mccouch S.Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)[J].Molecular & General Genetics:MGG,1996,252(5):597-607.
[15] Nei M,Li W H.Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76(10):5269-5273.
[16] 钟扬,陈家宽,黄德世.数量分类的方法与程序[M].武汉:武汉大学出版社,1990:47.
[17] 唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002:551-603.
[18] 王淼,于恒秀,龚志云,等.芍药栽培品种ISSR反应体系的优化和应用[J].扬州大学学报:农业与生命科学版,2007,28(2):78-82.
[19] 于恒秀,王淼,梁国华,等.ISSR引物鉴定芍药栽培品种之间亲缘关系的初步研究[J].植物生理学通讯,2006,42(2):271-274.
[20] Hao Q,Liu Z,Shu Q,et al.Studies on paeonia cultivars and hybrids identification based on SRAP analysis[J].Hereditas,2008,145(1):38-47.

Please choose a citation manager

Content to export