SSR Markers Linked of the Resistance Gene for Downy Mildew in Cucumis melo ssp.melo PI414723

doi:10.7668/hbnxb.2016.S1.015

Abstract

Abstract: In order to study the inheritance of wild melon PI414723,founding the molecular markers linked to resistance genes and locating the resistance genes.The F₂,BCr and BCs populations were derived by crossing and back crossing with Cucumis melo ssp. melo lines PI414723 and kalakesai.Investigation and statistical analyses scored for response to inoculation with[Pseudoperonospora cubensis (Berk.etCurt.) Rostov.].Based on the genetic linkage map that was constructed by the ICuGI.SSR analysis,combined with bulked segregation analysis(BSA),was done on the DNA of F₂ using 1 090 pairs of SSR primers.The results indicated that the resistance to downy mildew in PI414723 was controlled by one single recessive gene. An unique 226 bp fragment was amplified with the primer DM0231.It was identified that was linked to resistance gene of PI414723 at a distance of 2.67 cM.The resistance gene was located to LG9.This SSR marker DM0231could be useful for marker assisted selection in melon breeding for downy mildew resistance.

Key words: Cucumis melo ssp. melo, Downy mildew, Disease resistance gene, SSR marker

CLC Number:

Q78
S652.03

ZHANG Xuejun, YANG Juntao, LI Meihua, YANG yong, YI Hongping. SSR Markers Linked of the Resistance Gene for Downy Mildew in Cucumis melo ssp.melo PI414723[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 86-91. doi: 10.7668/hbnxb.2016.S1.015.

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