The Study of Genetic Relationship of Four Different Materials in Medicago L.

doi:10.7668/hbnxb.2016.S1.027

Abstract

Abstract: Morphology and ISSR Marker and their clusters analysis were carried out in 5 M. sativa L.,3 M. falcata L.,3 alfalfa with cream flower and 3 M. ruthenica L. for analysing the genetic relationship to further reveal the taxonomic relationship. The results showed alfalfa with cream flower had the significant differences in flower color(cream) and pod(1.0-1.5 coils). In cluster analysis,2 groups were clustered between 3 M. ruthenica L. and 11 Medicago L. which meant the significant at the genetic distance of 15;at the genetic distance of 10,3 groups were clustered in 5 M. sativa L.,3 M. falcate L. and 3 alfalfa with cream flower. From the relationship, M. ruthenica L. had the closer relationship than that of M. sativa L. and alfalfa with cream flower;alfalfa with cream flower had the closer relationship than that of M. sativa L.. At the genetic distance of 0.80,4 groups were clustered in 5 M. sativa L.,3 M. falcata L.,3 alfalfa with cream flower and M. ruthenica L. in ISSR Marker,by the genetic relationship,alfalfa with cream flower had the closer genetic relationship with M. sativa L. comparing with M. falcate L.,but, M. sativa L. had the equal genetic relationship with alfalfa with cream flower and with M. falcate L..

Key words: Medicago L., Morphology, ISSR Marker, Genetic relationship

CLC Number:

S551.03

LIU Lei, LI Zhiyong, SHI Wengui, LI Hongyan, ZHAO Jinmei, HUANG Fan, LI Jun. The Study of Genetic Relationship of Four Different Materials in Medicago L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 150-155. doi: 10.7668/hbnxb.2016.S1.027.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.S1.027

http://www.hbnxb.net/EN/Y2016/V31/IS1/150

References

[1] 魏臻武,盖钧镒. 豆科模式植物——蒺藜苜蓿[J]. 草业学报,2008,17(1):114-120.
[2] 卢欣石. 苜蓿属植物分类研究进展分析[J]. 草地学报,2009,17(5):680-685.
[3] 李飞飞,羊海军,崔大方. 利用SSR及ISSR分子标记研究苜蓿属及其近缘植物的亲缘关系[J]. 中山大学学报:自然科学版,2014,53(1):113-120.
[4] 内蒙古植物志编辑委员会. 第三卷[M]. 2版. 呼和浩特:内蒙古人民出版社,1989.
[5] 中国植物志编辑委员会.中国植物志[M].北京:科学出版社出版,1998
[6] Waldron L R. AN alfalfa bud mutation:a White-Flowered alfalfa branch found upon a Lavender-flowered plant[J]. Journal of Heredity,1925,10(16):423-424.
[7] Lesins K. Somatic flower color mutations in alfalfa[J]. Journal of Heredity,1956,47(4):171-179.
[8] 王红艳,贾志宽,韩清芳,等. 白花苜蓿单株生长特性的初步研究[J]. 西北农业学报,2006,15(3):190-192.
[9] 吴凤萍. 白花苜蓿生产性能、抗性及营养品质的初步研究[D]. 杨凌:西北农林科技大学,2008.
[10] 云锦凤,米福贵,杨青川,等.牧草育种技术[M].北京:化学工业出版社,2004
[11] Taia K W. Modern trends in plant taxonomy[J]. Asian Journal of Plant Sciences,2005,4(2):184-206
[12] 刘磊,王宗礼,李志勇,等. 基于ISSR分子标记的扁蓿豆植物分类学研究[J]. 中国草地学报,2015,37(3):26-30.
[13] 马志刚,吕作舟,郑和斌,等. ISSR标记在侧耳属菌株分类学中的初步应用[J]. 华中农业大学学报,2006,25(1):55-59.
[14] 龙珑. 川点霉与茎点霉两属真菌的分子系统分类学研究[D]. 南昌:江西农业大学,2013.
[15] 林杰斌,刘明德. SPSS10.0与统计模式构建[M]. 北京:科学出版社,2002.
[16] 王小丽,李志勇,李鸿雁,等. 种子老化对扁蓿豆种质遗传完整性变化的影响[J]. 中国草地学报,2010,32(6):52-57.
[17] 王瑜. 紫花苜蓿抗褐斑病基因的ISSR,SRAP以及AFLP分子标记研究[D]. 北京:中国农业科学院,2009.
[18] Sinskaya E N. Flora of cultivated plants of the U.S.S.R. XIII perennial leguminous[M]. Washington D C:National Science Foundation,1961.
[19] Lesins K,Lesins I. Genus medicago(Leguminasae):a taxogenetic study[J]. Vegetation,1979,50(2):92-92.
[20] Quiros C F,Bauchan G R. The genus Medicago and the origin of the Medicago sativa L. complex,p. 93-124,In A. A. Hanson,et al.,eds[M]. Alfalfa and alfalfa improvement,Madison,WI. 1988.
[21] Charles R G,Skrdla W H,Spencer H C. Gunn. Classification of Medicago sativa L. using Legume characters and flower colors[J]. Journal of Crystal Growth,1978,106(2-3):400-404.
[22] Muhammet AK RO LU. Population structure,genetic diversity,phylogenetic analyses,and association mapping of biofuel traits in wild diploid alfalfa(Medicago sativa L.)accessions[M]. Georgia:Doctoral dissertation from The University of Georgia,2009.
[23] Frank,Kelzer.The History of Grimm Alfalfa[M].Chaskn,Minnesota:Minnesota Historical Society,1957.
[24] Lefébure T,Douady C J,Gouy M,et al. Relationship between morphological taxonomy and molecular divergence within Crustacea:proposal of a molecular threshold to help species delimitation[J]. Molecular Phylogenetics and Evolution,2006,40(2):435-447.
[25] Sofy H I,Layla A M,Iman M A. Karyotypic diversity of some tilapia species[J]. Nat Sci,2008,6(1):19-27.

Please choose a citation manager

Content to export