Abstract: Black shank disease is one of the main diseases of tobacco production.The screening,identification of the disease resistance and genetic analysis of tobacco germplasm resources are the base of breeding for disease resistance. In this study,we used Physiological race 1 black shank to identify the resistance to black shank of 49 tobacco germplasm resources and used SSR markers to analysis genetic relationship. The results showed:Among them 24 were resistant,including dayeyongyan,G80,E9,MS212-8;7 were moderate resistant;6 were moderate susceptible;12 were susceptible;6 were high susceptible.The average effective number of alleles of 49 tobacco germplasm resources was 3.06(1.31),and the average Shannon's information index was 1.18(0.36);The 49 germplasm materials were classed into 4 groups at the position of coefficient of 0.66 by the 23 SSR markers. The first group was resistance variety K326;the second group included 6 germplasm resources;the third group included 4 susceptible germplasm resources;the fourth group included 38 germplasm resources. The most of resistance to black shank germplasm was clustered in the fourth group,suggesting the genetic basis of the resistance to black shank germplasm resources was narrow.

Key words: Tobacco, Black shank, SSR, Genetic relationship

CLC Number: 

• S572.03