Genetic Diversity of Ziziphus jujuba Mill.and Z.jujuba var. spinosa Based on SSR Markers

doi:10.7668/hbnxb.2015.02.027

Abstract

Abstract: To know the genetic diversity and the genetic relationship of Ziziphus jujuba Mill. and Z.jujuba var.spinosa germplasm resources, 7 pairs of SSR molecular markers were used to analyze the genetic diversity of 16 Ziziphus jujuba Mill.and 17 Z.jujuba var. spinosa in this experiment.The results showed that 56 alleles were amplified in 16 jujbe samples, and number of effective alleles (Ne)ranged from 3.798 to 10.000 and averaged at 6.953, Shannon's information index (I) was 1.984, expected heterozygosity (He) was 0.837.Moreover, 73 alleles were amplified in 17 wild jujube samples, Ne ranged from 3.273 to 11.840 and averaged at 7.398, I was 2.105, He was 0.843.So, genetic diversity of both of jujube and wild jujube were rich, and the wild jujube level of genetic diversity was higher than that of jujube.And, the GenALEx analysis showed that gene flow between populations exchanged frequently, and population genetic differentiation percentage (F_st) of jujube and wild jujube was 0.055, the average value of gene flow among populations (Nm) was 4.295.Furthermore, NTSYSpc cluster analysis showed that jujube and wild jujube could be divided into three groups of jujube, wild jujube and transition group using SSR molecular markers.

Key words: Ziziphus jujuba Mill., Z.jujuba var.spinosa, SSR, Genetic relationship

CLC Number:

S665.1

ZHANG Peng-fei, YU Dong-feng, LIU Ya-ling, SONG Mei-ling, ZHANG Ru, YONG Peng. Genetic Diversity of Ziziphus jujuba Mill.and Z.jujuba var. spinosa Based on SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 150-155. doi: 10.7668/hbnxb.2015.02.027.

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