Diversity of Cultivated Chestnuts and It's Close Relative Species Using Chloroplast SSRs

doi:10.7668/hbnxb.2015.02.026

Abstract

Abstract: The objective of the study is to elucidate the genetic relationship and genetic diversity of Castanea species.Based on the technology of chloroplast microsatellite markers, of total 24 cpSSR primer pairs screened, only four presented length polymorphism in the Castanea mollissima tested.We surveyed 6 species, altogether 56 materials with cpSSR markers in order to estimate genetic variation and genetic structure.In the castanea plants, average number of alleles (Na), effective number of alleles (Ne), expected heterozygosity (He) and Nei's were 3.25, 2.554, 0.606 and 0.320, respectively.Apparently, the parameters of cpSSR loci were much lower than those revealed by nuclear SSRs.Furthermore, the intra-specific genetic diversity was high but varied greatly among different species, the wild chestnut in China showed the highest genetic diversity, which one was rich in heredity and variation.The results have important implications of understanding the population genetics and evolutionary patterns in the genus Castanea and provide baseline data for formulating the conservation and management strategies.

Key words: Castanea mollissima BL., cpSSR DNA, Genetic diversity, Sequence

CLC Number:

Q781
P735

CHENG Li-li, HU Guang-long, SU Shu-chai, HUANG Wu-gang. Diversity of Cultivated Chestnuts and It's Close Relative Species Using Chloroplast SSRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 145-149. doi: 10.7668/hbnxb.2015.02.026.

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References

[1] 张宇和,王福堂,高新一.板栗[M].北京:中国林业出版社,1989:1-10.
[2] 张宇和,柳鎏,梁维坚.中国果树志-板栗榛子卷[M].北京:中国林业出版社,2005:8-17.
[3] Huang H W,Dane F,Jiang Z W.Inheritance and Diversity of PGI in Chestnut (Castanea)[J].Journal of Wuhan Botanical Research,1999,17(1):1-4.
[4] Goul o L,Valdiviesso T,Santana C,et al.Comparison between phenetic characterization using RAPD and ISSR markers and phenotypic data of cultivated chestnut (Castanea sativa Mill.) [J].Genetic Resources and Crop Evolution,2001,48:329-338.
[5] Marinoni D,Akkak A,Bounous G,et al.Development and characterization of microsatellite markers in Castanea sativa (Mill.)[J].Molecular Breeding,2003,11(2):127-136.
[6] Xu D H,Abe J,Kanazawa A,et al.Identification of sequence variations by PCR-RFLP and its application to the evaluation of cpDNA diversity in wild and cultivated soybeans[J].Theoretical and Applied Genetics,2001,102(5):683-688.
[7] Doyle J J,Du J I.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:13-15.
[8] Weising K,Gardner R C.A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms[J].Genome,1999,42:9-19.
[9] Sebastiani F,Carnevale S,Vendramin G G.A new set of mono- and dinucleotide chloroplast microsatellites in Fagaceae[J].Molecular Ecology Notes,2004,4:259-261.
[10] 黄武刚,程丽莉,周志军,等.板栗野生居群与栽培品种间叶绿体微卫星遗传差异初探[J].林业科学,2009,45(10):62-68.
[11] Francis C Y,Yang R C.POPGENE VERSION 1.31,Microsoft Window-based Freeware for Population Genetic Analysis.University of Alberta And Tim Boyle,Centre for International Forestry Research.Website fttp://ftp.microsoft.com/softlib/slfiles/hpgl.exe[OL].1999.
[12] Peakall R,Smouse P E.GenAlEx 5:Genetic Analysis in Excel Population Genetic Software for Teaching and Research[M].Canberna:Australian National University,2001.
[13] Thompson J,Higgins D,Gibson T.Clustal W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J].Nucl Acids Res,1994,22:4673-4690.
[14] Provan J,Soranzo N,Wilson N J,et al.Gene pool variation in Caledonian and European Scots pine (Pinus sylvestris L.) revealed by chloroplast simple sequence repeats[J].Proceedings of the Royal Society of London Series B,1998,265(1407):1697-1750.
[15] 郎萍,黄宏文.栗属中国特有种居群的遗传多样性及地域差异[J].植物学报,1999,41(6):651-657.
[16] 肖正东,何定华,何锡山,等.中国栗AC/GT微卫星的提取和多态性及对安徽省板栗品种的分析[J].植物分子育种,2005,3(4):509-514.
[17] Lira C F,Cardoso S R S,Ferreira C G,et al.Long-term population isolation in the endangered tropical tree species Caesalpinia echinata Lam.revealed by chloroplast microsatellites[J].Molecular Ecology.2003,12(12):3219-3225.
[18] Ellis J R,Pashley C H,Burke J M,et al.High genetic diversity in a rare and endangered sunflower as compared to a common congener[J].Molecular Ecology,2006,15(9):2345-2355.
[19] Nevo E,Beharav A,Meyer R C,et al.Genomic microsatellite adaptive divergence of wild barley by microclimatic stress in Evolution Canyon',Israel[J].Biological Journal of the Linnean Society,2005,84(2):205-224.

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