ACTA AGRICULTURAE BOREALI-SINICA ›› 2015, Vol. 30 ›› Issue (2): 140-144. doi: 10.7668/hbnxb.2015.02.025

Special Issue: Cucumber

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Identification of Nonbitter Germplasm by SSR Markers in Cucumber

ZHANG Li-jie3, MA Yan-chao1,2, LI Kang1, DONGFANG Yang2, WANG Jian-she1   

  1. 1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, China;
    2. College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;
    3. College of Agronomy, Ningxia University, Yinchuan 750021, China
  • Received:2015-02-11 Published:2015-04-28

Abstract: Identifying non-bitter germplasms and breeding non-bitter varieties are effective to control bitterness as well as to improve qualities in cucumber.In this study, SSR02309 marker linked to foliage bitter gene and SSR10795 marker linked to fruit bitter gene were used to identify the marker genotypes of 78 cucumber germplasm resources.The result showed genetic variation of marker genotypes exist among different germplasms and within same germplasms as well.The marker genotypes of 78 germplasms were confirmed, and 7 non-bitter cucumber varieties were preliminary identified by using the 2 markers.The sensory evaluation results by tasting such 7 cucumber varieties were consistent with the molecular identification results.This study laid technical and germplasm foundation for improving non-bitter cucumber varieties.

Key words: Cucumber, Foliage, Fruit, Bitter gene, SSR markers identification

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Cite this article

ZHANG Li-jie, MA Yan-chao, LI Kang, DONGFANG Yang, WANG Jian-she. Identification of Nonbitter Germplasm by SSR Markers in Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 140-144. doi: 10.7668/hbnxb.2015.02.025.

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