Transcriptomics Analysis of the Mechanism of Exogenous H2S Regulating Cucumber in Response to Salt Stress

doi:10.7668/hbnxb.20191173

Abstract

Abstract: In order to verify the mechanism of hydrogen sulfide to regulate the plant response to salt stress, cucumber seedlings were used as materials and treated with Hoagland(CK), 200 mmol/L NaCl(T) and 200 mmol/L NaCl+15 mmol/L NaHS (the donor of H₂S, S). The expression of transcriptome genes of cucumber roots were studied by using Illumina HiSeq high-throughput sequencing technology. A total of 365.35 Mb of Clean reads were obtained from 9 samples. Mapped reads was 315.74 Mb, and the percentage was 86.27%-88.64%. Analysis of differentially expressed genes revealed that T group had 1 168 genes up-regulated and 1 076 genes down-regulated compared with CK group, and S group had 435 genes up-regulated and 218 genes down-regulated compared with T group. The Gene Ontology (GO) enrichment analysis revealed that the differentially expressed genes were mainly concentrated in binding and catalytic activity in molecular functional classes; in biological process classes, they were mainly enriched in metabolic processes and cellular processes and single-organism process; Among cell components, the membrane and membrane part classes were mainly concentrated. KEGG enrichment analysis of the differentially expressed genes could be divided into 139, 75 and 127 pathway of 3 comparison groups, respectively. The differentially expressed genes in S group compared to the T group were mainly abundant during plant hormone signal transduction, protein processing in endoplasmic reticulum and photosynthesis pathway. Screening of 10 genes that might be related to H₂S regulating cucumber response to salt stress:CSC1-like protein ERD4, nitrate reductase (NADH)-like, protein TIFY 10B-like, allene oxide cyclase, BTB/POZ and TAZ domain-containing protein 1-like, tricalbin-3-like, mitogen-activated protein kinase kinase 3-like, transcription factor bHLH18-like, IAA-amino acid hydrolase ILR1-like 4 and potassium channel AKT1. Studying the mechanism of H₂S regulating cucumber response to salt stress provides theoretical data and reference basis for improving plant salt tolerance.

Key words: Cucumber, H₂S, NaCl stress, GO analysis, KEGG analysis

CLC Number:

Q78
S642.01

JIANG Jinglong, LI Li. Transcriptomics Analysis of the Mechanism of Exogenous H₂S Regulating Cucumber in Response to Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 17-25. doi: 10.7668/hbnxb.20191173.

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