Effects of Exogenous 2,4-epibrassinolide on Photosynthetic Characteristics of Oat Seedlings under NaCl Stress

doi:10.7668/hbnxb.20190663

Abstract

Abstract: To understand the regulation mechanism of exogenous 2,4-epibrassinolide (EBR) on photosynthesis of oats under NaCl stress, the effects of exogenous EBR on photosynthetic gas exchange parameters, fluorescence kinetic parameters, photosynthetic electron transfer rate and PSⅡ light energy distribution of oat seedlings were studied under 100 mmol/L NaCl stress with Avena sativa L. cv. Jiayan 2 and Qingyin 2 as experimental materials. The results showed that, NaCl stress significantly reduced the photosynthetic pigment content of oat seedlings, inhibited the activity of PSⅡ reaction center, and led to the decrease of PSⅡ photochemical efficiency and Net photosynthetic rate (Pn) of oat seedlings. Compared with NaCl stress, exogenous EBR treatments significantly increased the contents of chlorophyll a (Chla), chlorophyll b (Chlb) and carotenoids in the leaves of oat seedlings; significantly increased photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and water use efficiency (WUE),but decreased intercellular CO₂ concentration (Ci); exogenous EBR treatments also significantly increased maximum fluorescence (Fm), photochemical quenching (qP), photochemical quantum yield of photosystem II (ФPS Ⅱ), potential photochemical efficiency (Fv/Fo),maximal chemical efficiency (Fv/Fm), conversion efficiency of antenna pigment (Fv'/Fm'), photosynthetic electron transport rate (ETR), PSⅡ photochemical reaction energy (P), but reduced initial fluorescence (Fo) and non photochemical quenching coefficient (NPQ), antenna heat dissipation (D) and reaction center dissipation (E). In conclusion, exogenous EBR could significantly improve the actual photochemical efficiency of PSⅡ reaction center of oat seedlings under NaCl stress, effectively alleviate the photoinhibition and the degree of damage in the PSⅡ reaction center induced by NaCl stress, and improve the carbon assimilation efficiency and photosynthetic capacity of oat seedlings. These results indicated that exogenous BRs played a positive role in the regulation of the photosynthesis under salt stress, which could alleviate the damage caused by salt stress on oat seedlings.

Key words: Avena sativa L., NaCl stress, 2,4-epibrassinolide, Photosynthesis

CLC Number:

S634.01

KOU Jiangtao. Effects of Exogenous 2,4-epibrassinolide on Photosynthetic Characteristics of Oat Seedlings under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 79-87. doi: 10.7668/hbnxb.20190663.

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