Abstract:

GDSL is an important gene affecting the development of cuticle.This study cloned cucumber GDSL lipase gene and analyzed its expression pattern,in order to lay a foundation for the study of cuticle development and the glassiness of cucumber.The gene sequence was cloned to clarify its role in cucumbers with different glossiness by using six cultivars with different glossiness as materials.The promoter of CsGDSL lipase gene was cloned to analyze its functional elements.According to the reference sequence in cucumber genome database,we cloned the GDSL lipase gene of cucumber and analyzed by bio-informatics.The expression of the gene in different tissue parts of cucumber was confirmed by qRT-PCR technique.The CDS of CsGDSL length 1 059 bp which encoded 352 amino acids,and the secondary structure was mainly random curl(45.45%)and α-helix(33.24%).This gene was conservative in the process of evolution and was most closely related to CmGDSL.The expression level was the highest in the male flower of cucumber on the flowering day,and the expression level in the ovary at 3 days after flowering was higher than that at 0 days after flowering.The CDS sequence of CsGDSL gene was conservative in six cultivars. CsGDSL gene was responsive to stress,hormone and light.We obtained the CsGDSL lipase gene in this study,and identified its expression in different tissue parts of cucumber,the gene is relatively conserved in different cultivars,suggesting that CsGDSL may affect cucumber glossiness.

Key words: Cucumber, Cuticle, GDSL gene, Lipase