Screening of Strong Rhizosphere-colonizing Biocontrol Bacteria Based on Root Exudates and Their Application in Cucumber Fusarium Wilt Disease Management

doi:10.7668/hbnxb.20195721

Abstract

Abstract:

This study aims to establish a biocontrol bacteria screening system based on root exudates to screen biocontrol strains with strong rhizosphere colonization ability.Using this system,bacterial strains capable of synergistic control of cucumber Fusarium wilt in combination with spent mushroom substrate(SMS)were screened.The chemical composition of cucumber root exudates was modulated by pathogen stress and SMS induction.Chemotaxis,metabolic proliferation,and biofilm formation,which were colonization-related factors,were used as quantitative indicators for the tested strains.Efficient screening of rhizosphere-colonizing bacteria was achieved using capillary assays,96-well plate cultures,and biofilm formation tests.Among 200 bacterial strains isolated from the cucumber rhizosphere,significant differences were observed in chemotaxis,metabolic proliferation,and biofilm formation in response to root exudates.Using this screening system,Bacillus velezensis MTC-5 was screened as an outstanding strain across all screening indicators.Greenhouse experiments demonstrated that MTC-5 strain combined with SMS exhibited enhanced rhizosphere colonization ability and showed significant synergistic effects in disease suppression and plant growth promotion.Field trials revealed that the combined application of MTC-5 strain and SMS achieved a disease control efficacy of 74.5% against cucumber Fusarium wilt,which was 10.7,24.6 percent points higher than the efficacy of SMS or MTC-5 strain alone,respectively.It established a high-throughput biocontrol bacteria screening system based on root exudates,highlighting the critical role of root exudates in bacterial recruitment.The MTC-5 and SMS combination developed through this system demonstrated significant synergistic effects in controlling cucumber Fusarium wilt,providing new insights and technical support for biocontrol bacteria screening and application.

Key words: Biocontrol bacteria, Screening, Root exudates, Spent mushroom substrate, Cucumber Fusarium wilt, Bacillus

CLC Number:

QU Yuanhang, SU Zhenhe, DONG Lihong, ZHANG Xiaoyun, LI Shezeng, GUO Qinggang, MA Ping. Screening of Strong Rhizosphere-colonizing Biocontrol Bacteria Based on Root Exudates and Their Application in Cucumber Fusarium Wilt Disease Management[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 168-176. doi: 10.7668/hbnxb.20195721.

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Figures/Tables 7

References 29

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菌株 Strains	细胞密度(OD₆₀₀)Cell density		增幅/% Increase rate
菌株 Strains	CK-CRE	SMS-CRE	增幅/% Increase rate
MTC-5	0.049±0.007 3b	0.177±0.009 0a	261.22
GY2-27	0.080±0.011 1b	0.221±0.011 9a	176.25
MTS-35	0.060±0.008 0b	0.135±0.007 2a	125.00
MTS-29	0.067±0.007 7b	0.148±0.010 6a	120.90
R-10	0.064±0.007 2b	0.135±0.011 0a	110.94
MTC-3	0.063±0.009 2b	0.129±0.010 3a	104.76
LY2-15	0.077±0.009 3b	0.154±0.011 9a	100.00
GYS-17	0.071±0.006 9b	0.137±0.006 0a	92.96
LYS-28	0.072±0.008 3b	0.135±0.009 3a	87.50
MTC-2	0.063±0.008 2b	0.114±0.006 9a	80.95
R-8	0.075±0.006 1b	0.133±0.010 0a	77.33
MTS-13	0.067±0.007 5b	0.115±0.007 7a	71.64
GY2-16	0.068±0.009 8b	0.111±0.007 3a	63.24
MTC-20	0.069±0.007 1b	0.112±0.012 0a	62.32
R-9	0.181±0.009 1b	0.289±0.006 3a	59.67
GY2-28	0.080±0.011 9b	0.126±0.008 2a	57.50
MTC-6	0.078±0.010 4b	0.121±0.011 0a	55.13
LYS-14	0.093±0.010 1b	0.140±0.010 8a	50.54
LY2-5	0.105±0.009 3b	0.158±0.008 1a	50.48
MTC-1	0.073±0.010 1b	0.109±0.011 4a	49.32
GYS-3	0.086±0.008 3b	0.127±0.008 4a	47.67
GYS-34	0.097±0.007 2b	0.141±0.009 2a	45.36
GYS-19	0.075±0.008 9b	0.108±0.010 6a	44.00
GYS-22	0.077±0.008 3b	0.108±0.011 4a	40.26
MTC-25	0.081±0.006 6b	0.113±0.011 5a	39.51
LY2-2	0.103±0.009 7b	0.143±0.007 0a	38.83
R-1	0.110±0.007 5b	0.152±0.010 2a	38.18
GYS-21	0.115±0.009 6b	0.155±0.007 3a	34.78
GYS-28	0.106±0.009 5a	0.118±0.007 1a	11.32
GYS-32	0.104±0.008 7a	0.113±0.010 1a	8.65
GY2-4	0.107±0.006 9a	0.111±0.007 9a	3.74
LY2-1	0.104±0.010 9a	0.106±0.011 0a	1.92
GYS-5	0.117±0.009 8a	0.118±0.009 4a	0.85
MTS-3	0.137±0.009 8a	0.134±0.009 3a	-2.19
GYS-31	0.149±0.006 4a	0.142±0.010 1a	-4.70
LY2-19	0.120±0.009 4a	0.109±0.011 1a	-9.17
MTC-18	0.120±0.010 3a	0.107±0.008 3a	-10.83
LY2-16	0.127±0.011 4a	0.110±0.010 9a	-13.39
GYS-36	0.129±0.007 5a	0.109±0.008 8a	-15.50
MTC-29	0.123±0.008 0a	0.103±0.009 1a	-16.26
GYS-12	0.133±0.006 3a	0.109±0.010 6a	-18.05
MTS-33	0.129±0.009 1a	0.104±0.010 9b	-19.38
MTS-24	0.129±0.011 5a	0.100±0.007 1b	-22.48
GYS-2	0.139±0.009 5a	0.102±0.007 4b	-26.62
GY2-24	0.137±0.008 0a	0.100±0.008 5b	-27.01
MTC-22	0.142±0.011 4a	0.102±0.007 2b	-28.17
LYS-24	0.146±0.006 8a	0.101±0.011 0b	-30.82

菌株 Strains	细胞密度(OD₆₀₀)Cell density		增幅/% Increase rate
菌株 Strains	CK-CRE	SMS-CRE	增幅/% Increase rate
MTC-5	0.049±0.007 3b	0.177±0.009 0a	261.22
GY2-27	0.080±0.011 1b	0.221±0.011 9a	176.25
MTS-35	0.060±0.008 0b	0.135±0.007 2a	125.00
MTS-29	0.067±0.007 7b	0.148±0.010 6a	120.90
R-10	0.064±0.007 2b	0.135±0.011 0a	110.94
MTC-3	0.063±0.009 2b	0.129±0.010 3a	104.76
LY2-15	0.077±0.009 3b	0.154±0.011 9a	100.00
GYS-17	0.071±0.006 9b	0.137±0.006 0a	92.96
LYS-28	0.072±0.008 3b	0.135±0.009 3a	87.50
MTC-2	0.063±0.008 2b	0.114±0.006 9a	80.95
R-8	0.075±0.006 1b	0.133±0.010 0a	77.33
MTS-13	0.067±0.007 5b	0.115±0.007 7a	71.64
GY2-16	0.068±0.009 8b	0.111±0.007 3a	63.24
MTC-20	0.069±0.007 1b	0.112±0.012 0a	62.32
R-9	0.181±0.009 1b	0.289±0.006 3a	59.67
GY2-28	0.080±0.011 9b	0.126±0.008 2a	57.50
MTC-6	0.078±0.010 4b	0.121±0.011 0a	55.13
LYS-14	0.093±0.010 1b	0.140±0.010 8a	50.54
LY2-5	0.105±0.009 3b	0.158±0.008 1a	50.48
MTC-1	0.073±0.010 1b	0.109±0.011 4a	49.32
GYS-3	0.086±0.008 3b	0.127±0.008 4a	47.67
GYS-34	0.097±0.007 2b	0.141±0.009 2a	45.36
GYS-19	0.075±0.008 9b	0.108±0.010 6a	44.00
GYS-22	0.077±0.008 3b	0.108±0.011 4a	40.26
MTC-25	0.081±0.006 6b	0.113±0.011 5a	39.51
LY2-2	0.103±0.009 7b	0.143±0.007 0a	38.83
R-1	0.110±0.007 5b	0.152±0.010 2a	38.18
GYS-21	0.115±0.009 6b	0.155±0.007 3a	34.78
GYS-28	0.106±0.009 5a	0.118±0.007 1a	11.32
GYS-32	0.104±0.008 7a	0.113±0.010 1a	8.65
GY2-4	0.107±0.006 9a	0.111±0.007 9a	3.74
LY2-1	0.104±0.010 9a	0.106±0.011 0a	1.92
GYS-5	0.117±0.009 8a	0.118±0.009 4a	0.85
MTS-3	0.137±0.009 8a	0.134±0.009 3a	-2.19
GYS-31	0.149±0.006 4a	0.142±0.010 1a	-4.70
LY2-19	0.120±0.009 4a	0.109±0.011 1a	-9.17
MTC-18	0.120±0.010 3a	0.107±0.008 3a	-10.83
LY2-16	0.127±0.011 4a	0.110±0.010 9a	-13.39
GYS-36	0.129±0.007 5a	0.109±0.008 8a	-15.50
MTC-29	0.123±0.008 0a	0.103±0.009 1a	-16.26
GYS-12	0.133±0.006 3a	0.109±0.010 6a	-18.05
MTS-33	0.129±0.009 1a	0.104±0.010 9b	-19.38
MTS-24	0.129±0.011 5a	0.100±0.007 1b	-22.48
GYS-2	0.139±0.009 5a	0.102±0.007 4b	-26.62
GY2-24	0.137±0.008 0a	0.100±0.008 5b	-27.01
MTC-22	0.142±0.011 4a	0.102±0.007 2b	-28.17
LYS-24	0.146±0.006 8a	0.101±0.011 0b	-30.82

菌株 Strains	定殖量/(×10⁵ cfu/g) Colonization quality		增幅/% Increase rate
菌株 Strains	BAC	SMS-BAC	增幅/% Increase rate
MTC-5	8.61±0.11b	13.51±0.12a	57.20
GYS-21	8.28±0.42b	12.65±0.86a	52.30
MTC-25	8.22±0.62b	12.03±0.85a	46.30
MTC-20	9.54±0.67b	11.91±0.56a	24.40
LY2-5	7.41±0.47b	9.95±0.36a	34.30
GY2-16	8.87±0.67a	9.26±0.35a	4.40
R-10	6.39±0.87b	8.70±0.12a	36.20
GY2-27	5.71±0.89b	8.40±0.24a	47.30
GYS-17	7.63±0.53a	8.10±0.72a	6.20
GY2-28	6.20±0.38a	6.46±0.17a	4.10
MTS-13	5.13±0.22a	5.29±0.37a	3.10
GYS-19	2.89±0.04b	4.41±0.76a	52.40
R-9	1.66±0.12b	2.18±0.11a	31.00
LYS-14	0.69±0.02a	7.25±0.33a	4.40

菌株 Strains	定殖量/(×10⁵ cfu/g) Colonization quality		增幅/% Increase rate
菌株 Strains	BAC	SMS-BAC	增幅/% Increase rate
MTC-5	8.61±0.11b	13.51±0.12a	57.20
GYS-21	8.28±0.42b	12.65±0.86a	52.30
MTC-25	8.22±0.62b	12.03±0.85a	46.30
MTC-20	9.54±0.67b	11.91±0.56a	24.40
LY2-5	7.41±0.47b	9.95±0.36a	34.30
GY2-16	8.87±0.67a	9.26±0.35a	4.40
R-10	6.39±0.87b	8.70±0.12a	36.20
GY2-27	5.71±0.89b	8.40±0.24a	47.30
GYS-17	7.63±0.53a	8.10±0.72a	6.20
GY2-28	6.20±0.38a	6.46±0.17a	4.10
MTS-13	5.13±0.22a	5.29±0.37a	3.10
GYS-19	2.89±0.04b	4.41±0.76a	52.40
R-9	1.66±0.12b	2.18±0.11a	31.00
LYS-14	0.69±0.02a	7.25±0.33a	4.40

菌株 Strains	BAC		SMS-BAC		防效增幅/百分点 Efficiency enhancement
菌株 Strains	发病率/% Disease incidence	防病效果/% Control effect	发病率/% Disease incidence	防病效果/% Control effect	防效增幅/百分点 Efficiency enhancement
CK	87.60±7.02a	0.00	87.60±7.02a	0.00	0.00
LY2-13	70.41±5.43cde	19.62	59.05±5.51d	32.59	12.97
GY2-11	72.98±5.41bcd	16.69	57.01±6.39de	34.92	18.23
GYS-32	83.98±7.23ab	4.13	78.32±6.10ab	10.59	6.46
GYS-21	61.82±7.88def	29.43	32.38±6.19g	63.04	33.61
GYS-11	58.73±6.00ef	32.96	32.13±5.12g	63.32	30.36
GY2-1	79.63±5.64abc	9.10	63.67±5.06cd	27.32	18.22
LYS-17	51.08±6.51fg	41.69	19.87±4.80h	77.32	35.63
MTS-27	66.96±5.66cde	23.56	45.34±6.87ef	48.24	24.68
GYS-13	85.47±5.21a	2.43	72.52±4.41bc	17.21	14.78
MTC-5	46.51±4.13g	46.91	13.41±2.80h	84.69	37.78
LY2-3	65.79±6.38cde	24.90	40.83±3.17fg	53.39	28.49
MTC-28	71.19±7.35bcde	18.73	56.46±4.51de	35.55	16.82
LYS-13	86.65±7.12a	1.08	76.04±4.95ab	13.20	12.12
GY2-3	59.71±6.26ef	31.84	45.77±6.64ef	47.75	15.91
SMS	81.32±9.81abc	7.17	—	—	—

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