Screening of Lead Resistance Strains and Their Absorption Characteristics in Wheat Field Soil

doi:10.7668/hbnxb.2016.06.031

Abstract

Abstract: This study focuses on the lead tolerance and removal efficiency of microorganisms from the lead-contaminated soil.Two lead-resistant strains denoted by BY-10 and FB-6 were isolated from contaminated soil around a lead refinery.And the lead biosorption characteristics and optimal growth conditions of the strains were studied.The results showed that strains BY-10 and FB-6 belonged to Bacillus cereus and Penicillium commune respectively,and their tolerance concentrations to lead were 1 700,2 500 mg/L,respectively.In addition,the strains had a wide range of growing conditions.The optimal temperature of Bacillus cereus was 35℃ and that of Penicillium commune was 30℃.For both Bacillus cereus and Penicillium commune,the optimal growth pH was 7 and the liquid medium volume was 80-100 mL (in 250 mL bottle).With the increase of initial lead concentration from 0 to 600 mg/L, the growth of two strains became slower and slower gradually,while the tolerance of Penicillium commune to lead was stronger than that of Bacillus cereus when the lead concentration was over 400 mg/L.At a certain concentration of lead,the lead adsorption rate of the two strains increased firstly and then decreased gradually with the increasing growth time of strains.The dynamic change of biological adsorption quantity of lead by the two strains was similar to the adsorption rate of lead.But in a certain growth time of strains,there was a descending trend in the adsorption rate of strains to lead and an ascending trend in the biological adsorption quantity of them with the increase of lead concentration.Furthermore,the lead adsorption rate of Bacillus cereus and Penicillium commune reached the top at the 150 mg/L concentration of lead,and their values were 66.25% and 74.88% respectively.

Key words: Wheat field soil, Lead resistance strains, Screening and identification, Growing conditions, Absorption

CLC Number:

S154
S512.01

SHAO Yun, HAO Zhenzhen, WANG Hailei, LI Xiangli, WANG Wenfei, CHEN Jingwen, WANG Jingruo. Screening of Lead Resistance Strains and Their Absorption Characteristics in Wheat Field Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 199-205. doi: 10.7668/hbnxb.2016.06.031.

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