Related Genes of Cucumber in vitro Gynogenesis in the Early Stage from the Perspective of Transcriptome

doi:10.7668/hbnxb.20190589

Abstract

Abstract: Differentially expressed genes of cucumber in vitro gynogenesis in the early stage were analyzed by comparing the transcriptome of early cultured ovaries from both high and low regeneration frequency genotypes with the method of gene chips and qPCR technology. The results showed that 8 differentially expressed genes we selected, which appeared high level expressions in high regeneration frequency genotype, but low level expressions or overall decline in the low regeneration frequency genotype from 0th to 6th day of culture. 3 genes were found to be associated with hormonal stimulation response:respectively response to salicylic acid and jasmonic acid defense system, abscisic acid stimulation, auxin-induced binding protein and CTK expression in the Zeatin biosynthesis. 2 genes were found to be involved in the expression of POD activity which played an important role in the progress of biological oxidation. The remaining 3 genes were related to STK activity, aquaporin, mechanosensitive ion channel protein and separately participated in response to environmental stress, response to borates, arsenites and other boron, arsenic materials, transmit signals inside and outside cells and other biological processes.It is preliminarily believed that the 8 genes were important during the early stage of cucumber in vitro gynogenesis.

Key words: Cucumber, In vitro gynogenesis, Differentially expressed genes

CLC Number:

S642.03
Q78

WEI Aimin, YAO Yao, LIU Nan, CHE Liming, WEI Panpan, HAN Yike, CHEN Zhengwu, DU Shengli. Related Genes of Cucumber in vitro Gynogenesis in the Early Stage from the Perspective of Transcriptome[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 63-73. doi: 10.7668/hbnxb.20190589.

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http://www.hbnxb.net/EN/Y2019/V34/I6/63

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