Isolation and Identification of Maize ear rot Pathogens and Phylogenetic Analysis of Dominant Species in Shandong Province

doi:10.7668/hbnxb.201751508

Abstract

Abstract: The objective of this study was to determine the composition of pathogen causing Maize ear rot in Shandong Province and to analyze the epidemic trend of dominant pathogen. A total of 138 Maize ear rot samples were collected from Shandong Province during 2015-2017 and were used for pathogen isolation.The isolates were identified by morphological and molecular biology methods, and the occurrence regularity of dominant pathogen was clarified by phylogenetic analysis. The results of isolation and identification showed that a total of 18 fungi were obtained. The isolation frequency of Fusarium verticillioides was 67.39%, which was the dominant pathogen in Shandong Province, followed by F. proliferatum, F.graminearum, F.equiseti and F.oxysporium with separation frequencies of 10.87%, 7.97%, 5.80% and 0.72%, respectively; And the separation frequencies of Trichoderma harzianum, Penicillium oxalicum, Aspergillus flavus and A. niger were 7.91%, 6.52%, 2.17% and 5.07%, respectively. Based on the EF-1α gene sequence, the phylogenetic tree of F. verticillioides isolates in Shandong Province from 2015 to 2017 was constructed, and the genetic evolution relationship of isolates between Shandong Province and Hebei Province was analyzed. Phylogenetic analysis showed that 89 isolates of F. verticillioides could be divided into two large groups with a small genetic distance of 0.002-0.016, indicating that the genetic relationship between these isolates was relatively close. The genetic distance of F. verticillioides isolates in Shandong Province and Hebei Province was small and showed a close genetic relationship, indicating that there were frequent gene exchanges. The results of this study would provide a scientific basis for resistance breeding and integrated control of F. verticillioides.

Key words: Maize ear rot, Fusarium verticillioides, Separation frequency, Genetic relationship

CLC Number:

S435.131

DING Mengjun, YANG Yang, SUN Hua, MA Hongxia, LIU Shusen, SHI Jie. Isolation and Identification of Maize ear rot Pathogens and Phylogenetic Analysis of Dominant Species in Shandong Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 216-223. doi: 10.7668/hbnxb.201751508.

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