ACTA AGRICULTURAE BOREALI-SINICA ›› 2015, Vol. 30 ›› Issue (S1): 55-60. doi: 10.7668/hbnxb.2015.S1.010

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Analysis on Pollen Morphology and Variation of Viability of Lilium Asiatic Hybrids with Different Ploidy

CUI Guang-fen, DU Wen-wen, DUAN Qing, JIA Wen-jie, WANG Xiang-ning   

  1. Flower Institute of Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Yunnan Key Laboratory for Flower Breeding, Kunming 650205, China
  • Received:2015-09-09 Published:2015-12-28

Abstract: In order to investigate differences of pollen morphology and variation of viability of Lilium Asiatic hybrids in ploidy level,with 8 Asiatic lily cultivars as materials,pollen viability was measured by the methods of mixed dye staining and germination.Meanwhile,pollen morphology was observed with an electron microscope.The results showed that the highest pollen viability of three kinds of ploidy Asiatic lily appeared in the first day of the flowering period,which of the triploid varieties were at 10:30 AM,diploid and tetraploid cultivars were at 12:30 PM.Trends of pollen viability in 8 lily cultivars increased first and then decreased.There was a positive correlation between pollen size and ploidy.The polar axis ranged in 96.47-137.91 μm and the equatorial axis value were 40.68-56.55 μm.P×E value of tetraploid pollen was the largest,followed by triploid and diploid.The pollen central mesh size range 3.93 to 8.57 μm,which increased with increasing ploidy of varieties.Morphological parameters cluster displayed higher pollen similarity in the same ploidy hybrids.The research showed that there was a big difference in the persistence of pollen viability between different ploidy of Asiatic lilies.According to the ploidy of varieties,breeders can carry out pollination in the period of peak viability of pollen.

Key words: Asiatic lily, Varieties, Ploidy, Pollen viability, Pollen morphology

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Cite this article

CUI Guang-fen, DU Wen-wen, DUAN Qing, JIA Wen-jie, WANG Xiang-ning. Analysis on Pollen Morphology and Variation of Viability of Lilium Asiatic Hybrids with Different Ploidy[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 55-60. doi: 10.7668/hbnxb.2015.S1.010.

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