1Dx5 Genetic Transformation and Analysis in 26 New Winter Wheat

doi:10.7668/hbnxb.2016.S1.026

Abstract

Abstract: In order to carry out genetic analysis of T₁ generation of transgenic wheat with 1Dx5 gene,the Xinjiang winter common wheat Xindong No.26 were transformed with high-molecular-weight glutenin subunits (HMW-GS) gene 1Dx5,detected by SDS-PAGE,was found expression of 1Dx5 gene in 37 wheat seeds at the protein level.After subculture cultivation,37 T₁ generation transgenic lines were obtained.SDS-PAGE analysis of T₁ generation transgenic wheat seeds,the results showed that the composition of HMW-GS were found three phenotypes at protein level,phenotypic Ⅰ,the foreign 1Dx5 gene did not express;phenotypic Ⅱ,the foreign 1Dx5 gene express;phenotypic Ⅲ,endogenous 1Dx2 gene did not express.The probability of these three phenotypes were approximately 34.3%,45.9% and 19.8%,respectively.The study successfully obtained new types of HMW-GS composition,providing molecular evidence for the selection of high quality wheat.

Key words: Wheat, High quality, SDS-PAGE, HMW-GS

CLC Number:

WANG Cuifang, YANG Kun, ZENG Weijun, ZHU Changqing. 1Dx5 Genetic Transformation and Analysis in 26 New Winter Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 146-149. doi: 10.7668/hbnxb.2016.S1.026.

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