Identification of Pathogenic Nematode Isolated from Foxtail Millet in Hebei Province

doi:10.7668/hbnxb.2015.06.027

Abstract

Abstract: To identify a nematode isolate from infected ears of millet in Hebei province,the morphological characteristics and measurements of the nematode isolate were acquired and compared,its 28S rRNA-D2/D3 and ITS regions of ribosomal RNA were amplified,sequenced and aligned,and the phylogenetic trees were constructed by Neighbor-joining method using MEGA6.0 software.The results showed:this population was very similar to other Aphelenchoides besseyi populations reported before;compared with the sequences from GenBank,the 28S rRNA-D2/D3 regions of the nematode isolate was 100% similar to Aphelenchoides besseyi and the similarity of ITS was 98%-99%.The NJ trees showed that the nematode isolate was clustered into one branch with Aphelenchoides besseyi with 100% support rate.All the information revealed the nematode isolate from infected ears of millet in Hebei province should be Aphelenchoides besseyi.

Key words: Millet nematode disease, Symptom, Aphelenchoides besseyi, Morphology, Phylogenetic analysis, DNA barcoding

CLC Number:

S435.15

WANG Jin-cheng, LIN Yu, GU Jian-feng, DONG Li, GUO Jing-ze, CHEN Xian-feng, LI Zhi-yong. Identification of Pathogenic Nematode Isolated from Foxtail Millet in Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 176-181. doi: 10.7668/hbnxb.2015.06.027.

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http://www.hbnxb.net/EN/Y2015/V30/I6/176

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