Prevalence and Phylogenetic Analysis of Multiple Porcine bocavirus Genotypes in Clinically Healthy Pigs in Hunan Province

doi:10.7668/hbnxb.2017.05.021

Abstract

Abstract: To evaluate the prevalence of PBoV in healthy pig in different age,primers targeting to partial NS1 gene of PBoV1/2 and VP1 gene of PBoV3/4/5 were designed based on the analysis of PBoV sequences from GenBank.Total 430 anal swab species were collected from 5 pig farms with the pig'age of 2-20 weeks from August to December 2016 in Hunan Province.The total positive rate of PBoV was 64.6% (278/430),PBoV1/2 for 27.2%(117/430),PBoV3/4/5 for 45.1%(194/430) and with 7.7%(33/430) positive rate of coinfection.The mainly infection age of PBoV1/2 ranged 2-10 weeks,and PBoV3/4/5 from 8-16 weeks.The average nucleotide identity of obtained sequences and references of PBoV1/2 and PBoV3/4/5 was 85.4% and 67.2%,respectively.High prevalence and coinfection were observed in clinically healthy pigs,which may cause potential effect to the microbes in the gut.

Key words: PBoV, Prevalence, Phylogenetic analysis, Coinfection, Pathogenicity

CLC Number:

S855.3

QU Tailong, LI Runcheng, LUO Binyu, GE Meng, YAN Meijun, HU Huican, YU Xinglong. Prevalence and Phylogenetic Analysis of Multiple Porcine bocavirus Genotypes in Clinically Healthy Pigs in Hunan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 136-141. doi: 10.7668/hbnxb.2017.05.021.

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http://www.hbnxb.net/EN/Y2017/V32/I5/136

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