Analysis of Mutant Gene in BCt98 Mutant Enhanced Pathogenicity of Botrytis cinerea

doi:10.7668/hbnxb.2015.03.007

Abstract

Abstract: The objective of this study was to obtain pathogenicity-related genes of Botrytis cinerea and to investigate the function of pathogenicity-related genes.An enhanced pathogenicity mutant,named BCt98,was found by screening T-DNA insertional mutant library of Botrytis cinerea and it was testified by PCR and Southern Blotting techniques.T-DNA insertion site was defined in the third exon of BC1G_07014.1 gene by using TAIL-PCR and bioinformatics methods.The mutant gene was identified as BC1G_07014.1 by RT-PCR technology.Compared to the wild type strain,the mutant BCt98 growed quickly,colony was white,did not produce conidium and sclerotia,but showed stronger on cell wall degrading enzyme activity and toxin activity.These results showed that the BC1G_07014.1 gene was involved in growth,development,pathogenicity and involved in regulating cell wall degradation enzyme activity and toxin activity in B.cinerea.

Key words: Botrytis cinerea, Mutant BCt98, Pathogenicity, Mutant gene

CLC Number:

S432.1
Q78

WANG Min, DONG Li-ping, ZHAO Bin, ZHENG Xu, SI He-long, ZHANG Jing, SHI Cui-ping, XING Ji-hong, DONG Jin-gao. Analysis of Mutant Gene in BCt98 Mutant Enhanced Pathogenicity of Botrytis cinerea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 37-41. doi: 10.7668/hbnxb.2015.03.007.

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http://www.hbnxb.net/EN/Y2015/V30/I3/37

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