Abstract: Salmonella is a zoonotic important foodborne pathogen，a sensitive dual-PCR detection method was established is beneficial to achieve the detection of pathogenic Salmonella rapidly． In this thesis， the pathogenicity island genes of pathogenic Salmonella were as the research object and two pairs of primers which named Salmonella pathogenicity island mgtC and sopB were designed and synthesized based on the sequence of the Salmonella pathogenicity island genes． The nucleic acid of Salmonella typhimurium ( ATCC9150) was screened as a template，after primer specificity test， sensitivity test of DNA template and optimize the reaction conditions， the double PCR method of rapid differential detection for pathogenic Salmonella was successfully established． Specific test results showed that the primers mgtC and sopB of double PCR only can amplify two specific fragments of the Salmonella typhimurium ( ATCC9150) whose size is 500 bp and 1 000 bp， respectively． The sensitivity results showed that the limit of detection was 10 cfu /mL ． This double PCR method established could identify the virulence factor in Salmonella，which can achieve the detection of pathogenic Salmonella rapidly in high specificity and sensitivity．

Key words: Pathogenicity island, Gene mgtC, Gene sopB, Double PCR, Salmonella typhimurium

CLC Number: 

• S432.4