Identification of QTLs Associated with Complex Salt-alkaline Tolerance at the Seedling Stage in Sorghum

doi:10.7668/hbnxb.2015.03.008

Abstract

Abstract: Aimed to identify QTLs associated with seedling four traits under complex salt-alkaline stress in Sorghum and provide target loci and linked molecular markers for marker assisted selection of Sorghum salt-alkaline tolerant-related traits,an F₂ population with 170 lines derived from cross between BJ-299×Tx622B were used in this study.Quantitative trait loci (QTLs) for relative livability (RL),relative dry weight (RDW),relative fresh weight (RFW) and relative seedling height (RSH) at seedling stage were analyzed by interval mapping method.The results demonstrated that salt-alkaline tolerance at seedling stage of sorghum was a complex quantitative trait and controlled by multiple genes.Correlation coefficients between each two traits in the F_{2: 3} populations under complex salt-alkaline stress condition showed significant positive correlation.A total of 3 QTLs (qSAT-A, qSAT-D and qSAT-J) located on three major chromosome regions (Sam27281-Sam22486, Sam11433-Sam78379 and Sam62346a- Sam50778) on chromosome A,D and J,respectively.The negative effects were all from the salt-alkaline sensitive parent Tx622B. qSAT-A and qSAT-D were controlling four salt-alkaline tolerant related traits simultaneously,and explained phenotypic variation ranging from 8.0% to 18.8%.QTL qSAT-J for RL was detected with phenotypic variation explained of 7.3%.The two major QTLs (qSAT-A, qSAT-D) and three chromosome regions played crucial role in salt-alkaline tolerance of sorghum,which may have a potential in the practice of marker assisted sorghum breeding for improving salt-alkaline tolerance.

Key words: Sorghum, Seedling stage, Salt-alkaline tolerance, QTL mapping, SSR marker

CLC Number:

S514.03

TANG Chao-chen, GAO Jian-ming, HAN Yun, LUO Feng, PEI Zhong-you, SUN Shou-jun. Identification of QTLs Associated with Complex Salt-alkaline Tolerance at the Seedling Stage in Sorghum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 42-47. doi: 10.7668/hbnxb.2015.03.008.

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