Lily Mottle Virus Genome of Dalian Isolates Sequencing and Its Analysis

doi:10.7668/hbnxb.2013.S1.003

Abstract

Abstract: According to Lily mottle virus (LMoV) genome sequence logged in the GenBank,we seven pairs of overlapping primers,and use RT-PCR technique to detect Lilium Longiflorum White Heaven,then obtain the whole-genome sequences of Lily mottle virus of Dalian isolates (LMoV-DL) by cloning and sequencing. Through the sequence analysis indicates that: LMoV genome is the linear positive-sense single-stranded RNA which is constituted with the length of 9648 by bp comprising a poly (A) tail and encoding a molecular weight of 351 kDa poly-protein which is composed of 3096 amino acids. Poly-protein phylogeny analysis that the LMoV is a large branch of Potyvirus and close to BYMV and C1YVV on the evolutionary relationship.

Key words: Lily mottle virus, RT-PCR, Gene sequencing, Phylogenetic analysis

CLC Number:

S682.2
Q785

XU Pin-san, SUN Bing-lun, JIANG Xu, LI Huan-gai. Lily Mottle Virus Genome of Dalian Isolates Sequencing and Its Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 12-15. doi: 10.7668/hbnxb.2013.S1.003.

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